HAI-2/SPINT2 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HAI-2/SPINT2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HAI-2A
  Ala28-Lys197
  Accession # O43291
• Specificity: Detects human HAI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human HAI‑1 and recombinant mouse HAI-2B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPINT2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-2/SPINT2 Antibody

• HAI2
• HAI-2
• HAI-2DIAR3
• HAI2FLJ45571
• Hepatocyte growth factor activator inhibitor type 2
• Kop
• kunitz-type protease inhibitor 2
• PB
• Placental bikunin
• serine peptidase inhibitor, Kunitz type, 2
• serine protease inhibitor, Kunitz type, 2

Background

Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1-5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa. Recombinant HAI-2A prolonged the clotting time in an activated partial thromboplastin time assay.

1. Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
2. Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
3. Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
4. Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
5. Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAI-2/SPINT2 Antibody (AF1106)(4)

Publications using AF1106

• Li-Mei Chen, Karl X. Chai Exosome-Mediated Activation of the Prostasin-Matriptase Serine Protease Cascade in B Lymphoma Cells Cancers (Basel) 2023-07-28 [PMID: 37568664]
• CE Martin, AS Murray, KE Sala-Hamri, JR Mackinder, EC Harrison, JG Lundgren, FA Varela, K List Post-translational modifications of serine protease TMPRSS13 regulate zymogen activation, proteolytic activity, and cell surface localization The Journal of Biological Chemistry, 2021-09-22;0(0):101227. 2021-09-22 [PMID: 34562451] (Western Blot, Human)
• AS Murray, FA Varela, TE Hyland, AJ Schoenbeck, JM White, LM Tanabe, SV Todi, K List Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization J. Biol. Chem., 2017-07-14;0(0):. 2017-07-14 [PMID: 28710277] (Western Blot, Human)
• Erik W. Martin, Marguerite S. Buzza, Kathryn H. Driesbaugh, Shihui Liu, Yolanda M. Fortenberry, Stephen H. Leppla et al. Targeting the membrane-anchored serine protease testisin with a novel engineered anthrax toxin prodrug to kill tumor cells and reduce tumor burden Oncotarget 2015-10-20 [PMID: 26392335]

Bioinformatics

• Gene Symbol: SPINT2
• Uniprot:
  • O43291()