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![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human cerebral cortex, kidney, liver and pancreas using Anti-Phenylalanine Hydroxylase antibody NBP2-48615 (A) shows similar protein distribution across tissues to independent antibody NBP1-80917 (B).](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0014.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP2-48615-img0017.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0016.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0003.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0013.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-48615-img0015.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human cerebral cortex, kidney, liver and pancreas using Anti-Phenylalanine Hydroxylase antibody NBP1-80917 (A) shows similar protein distribution across tissues to independent antibody NBP2-48615 (B).](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0021.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Analysis using Anti-PAH antibody NBP1-80917 (A) shows similar pattern to independent antibody NBP2-48615 (B).](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP1-80917-img0013.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0025.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0019.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human kidney shows strong cytoplasmic positivity in cells in proximal tubules.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0022.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-80917] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-80917-img0023.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody (JE64-47) [NBP3-32708] - Western blot analysis of Phenylalanine Hydroxylase on rat liver tissue lysates with Rabbit anti-Phenylalanine Hydroxylase antibody (NBP3-32708) at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32708) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32708_rabbit-phenylalanine-hydroxylase-mab-je64-47-278202415295562.jpg "Western Blot: Phenylalanine Hydroxylase Antibody (JE64-47) [NBP3-32708] - Western blot analysis of Phenylalanine Hydroxylase on rat liver tissue lysates with Rabbit anti-Phenylalanine Hydroxylase antibody (NBP3-32708) at 1/500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32708) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Phenylalanine Hydroxylase Antibody (JE64-47) [NBP3-32708] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Phenylalanine Hydroxylase antibody (NBP3-32708) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32708) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32708_rabbit-phenylalanine-hydroxylase-mab-je64-47-298202414304170.jpg)
![Flow Cytometry: Phenylalanine Hydroxylase Antibody (JE64-47) [NBP3-32708] - Flow cytometric analysis of HepG2 cells labeling Phenylalanine Hydroxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32708, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32708_rabbit-phenylalanine-hydroxylase-mab-je64-47-29202416453238.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody (C4) [NBP3-41889] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41889; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41889_mouse-phenylalanine-hydroxylase-mab-c4-61220242151867.jpg "Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody (C4) [NBP3-41889] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30ug/ml NBP3-41889; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Phenylalanine Hydroxylase Antibody (C4) [NBP3-41889] - Sample: Lane1: Human Serum; Lane2: Rat Liver lysate; Lane3: Mouse Gallbladder lysate; Lane4: Hela cell lysate Primary Ab: 2ug/ml NBP3-41889; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41889_mouse-phenylalanine-hydroxylase-mab-c4-612202421175996.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP3-35577] - Western blot analysis of lysates from HepG2 cells, using Phenylalanine Hydroxylase Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35577_rabbit-phenylalanine-hydroxylase-pab-1012202411343395.jpg "Western Blot: Phenylalanine Hydroxylase Antibody [NBP3-35577] - Western blot analysis of lysates from HepG2 cells, using Phenylalanine Hydroxylase Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: Phenylalanine Hydroxylase Antibody [NBP3-35577] - Immunofluorescence analysis of HepG2 cells using Phenylalanine Hydroxylase Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35577_rabbit-phenylalanine-hydroxylase-pab-1012202411334084.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP1-52084-img0002.jpg "Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-NBP1-52084-img0001.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of PAH expression in human liver.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-H00005053-B01P-img0002.jpg "Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of PAH expression in human liver.")
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of purified antibody to PAH on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-H00005053-B01P-img0001.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody [H00005053-B01P] - Analysis of PAH expression in transfected 293T cell line by PAH polyclonal antibody. Lane 1: PAH transfected lysate(49.72 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-Western-Blot-H00005053-B01P-img0003.jpg)
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody (C11) [NBP3-41890] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml NBP3-41890; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41890_mouse-phenylalanine-hydroxylase-mab-c11-612202421195642.jpg "Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody (C11) [NBP3-41890] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml NBP3-41890; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Phenylalanine Hydroxylase Antibody (C11) [NBP3-41890] - Sample: Human Serum Primary Ab: 2ug/ml NBP3-41890; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41890_mouse-phenylalanine-hydroxylase-mab-c11-612202421283824.jpg)
![Immunohistochemistry: Phenylalanine Hydroxylase Antibody (17D10) [NBP3-26443] - Image of Phenylalanine Hydroxylase Antibody (17D10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26443_rabbit-phenylalanine-hydroxylase-mab-17d10-262202415475628.jpg "Immunohistochemistry: Phenylalanine Hydroxylase Antibody (17D10) [NBP3-26443] - Image of Phenylalanine Hydroxylase Antibody (17D10) diluted at 1:100 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: Phenylalanine Hydroxylase Antibody (17D10) [NBP3-26443] - Staining of MCF-7 cell with Phenylalanine Hydroxylase Antibody (17D10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 598-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26443_rabbit-phenylalanine-hydroxylase-mab-17d10-282202412493628.jpg)
![Western Blot: Phenylalanine Hydroxylase Antibody (S06-3H4) [NBP3-19516] - Western blot detection of Phenylalanine Hydroxylase in 3T3, C6, Rat Brain, K562 cell lysates using NBP3-19516 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/Phenylalanine-Hydroxylase-Antibody-S06-3H4-Western-Blot-NBP3-19516-img0001.jpg "Western Blot: Phenylalanine Hydroxylase Antibody (S06-3H4) [NBP3-19516] - Western blot detection of Phenylalanine Hydroxylase in 3T3, C6, Rat Brain, K562 cell lysates using NBP3-19516 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")
![Western Blot: Phenylalanine Hydroxylase Antibody (C3) [NBP3-41772] - Sample: Lane1: Rat Liver lysate; Lane2: Mouse Gallbladder lysate Primary Ab: 2ug/ml NBP3-41772; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41772_mouse-phenylalanine-hydroxylase-mab-c3-61220242058877.jpg "Western Blot: Phenylalanine Hydroxylase Antibody (C3) [NBP3-41772] - Sample: Lane1: Rat Liver lysate; Lane2: Mouse Gallbladder lysate Primary Ab: 2ug/ml NBP3-41772; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody (C10) [NBP3-41972] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml NBP3-41972; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41972_mouse-phenylalanine-hydroxylase-mab-c10-61220242171322.jpg "Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody (C10) [NBP3-41972] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 20ug/ml NBP3-41972; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")