Phenylalanine Hydroxylase Antibody (S06-3H4)

Western Blot: Phenylalanine Hydroxylase Antibody (S06-3H4) [NBP3-19516] - Western blot detection of Phenylalanine Hydroxylase in 3T3, C6, Rat Brain, K562 cell lysates using NBP3-19516 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S06-3H4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Phenylalanine Hydroxylase Antibody (S06-3H4) Summary

• Description: Novus Biologicals Rabbit Phenylalanine Hydroxylase Antibody (S06-3H4) (NBP3-19516) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Phenylalanine Hydroxylase (Uniprot # P00439)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PAH
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/5000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Phenylalanine Hydroxylase Antibody (S06-3H4)

• EC 1.14.16
• EC 1.14.16.1
• PH
• phe-4-monooxygenase
• phenylalanine 4-monooxygenase
• phenylalanine hydroxylase
• phenylalanine-4-hydroxylase
• PKU
• PKU1

Background

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAH