PGM1 Antibody (CL3301) - BSA Free

Western Blot: PGM1 Antibody (CL3301) [NBP2-59026] - Analysis in human cell line RT-4.

Immunohistochemistry-Paraffin: PGM1 Antibody (CL3301) [NBP2-59026] - Staining of human fallopian tube shows absence of immunoreactivity as expected (negative control).

Immunohistochemistry-Paraffin: PGM1 Antibody (CL3301) [NBP2-59026] - Staining of human liver shows strong immunoreactivity in hepatocytes.

Immunohistochemistry-Paraffin: PGM1 Antibody (CL3301) [NBP2-59026] - Staining of human small intestine shows cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL3301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

PGM1 Antibody (CL3301) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P36871, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for PGM1 Antibody (CL3301) - BSA Free

• CDG1T
• EC 5.4.2
• EC 5.4.2.2
• Glucose phosphomutase 1
• GSD14
• PGM 1
• PGM1
• phosphoglucomutase 1
• phosphoglucomutase-1

Background

Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 (PubMed 8257433)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGM1