PGM1 Antibody (CL3301) - Azide and BSA Free

Staining of human fallopian tube shows absence of immunoreactivity as expected (negative control).

Staining of human liver shows strong immunoreactivity in hepatocytes.

Analysis in human cell line RT-4.

Staining of human small intestine shows cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3301
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PGM1 Antibody (CL3301) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PGM1 Antibody (CL3301) - Azide and BSA Free (NBP2-59026) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P36871, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for PGM1 Antibody (CL3301) - Azide and BSA Free

• CDG1T
• EC 5.4.2
• EC 5.4.2.2
• Glucose phosphomutase 1
• GSD14
• PGM 1
• PGM1
• phosphoglucomutase 1
• phosphoglucomutase-1

Background

Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 (PubMed 8257433)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGM1