DLC1 Antibody - Isoform 1 and 3

Images

 
Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Negative Control showing staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.
Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Staining of paraffin embedded Mouse Adrenal Gland. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary ...read more
Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DLC1 Antibody - Isoform 1 and 3 Summary

Immunogen
Peptide with sequence SVAIRKRSWEEHC corresponding to N-Terminus according to NP_872584.2, NP_079043.3.
Specificity
This antibody is expected to recognise isoforms 1 and 3 ( NP_872584.2 and NP_079043.3 resp) but NOT isoform 2 (NP_006085.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DLC1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 ug/mL
  • Immunohistochemistry-Paraffin 4 ug/mL
  • Immunofluorescence 10 ug/mL
Application Notes
WB successful usage for human heart is reported in scientific literature (PMID: 19874489). IHC-P: Human adrenal gland (medulla) shows strong reticulate staining in the cytoplasm of medular cells.
Control
DLC1 Overexpression Lysate
Publications
Read Publications using NB300-917.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DLC1 Antibody - Isoform 1 and 3

  • ARHGAP7 STARD12 deleted in liver cancer 1 variant 2
  • Deleted in liver cancer 1 protein
  • deleted in liver cancer 1
  • DLC-1
  • FLJ21120
  • HP protein
  • HP
  • KIAA1723
  • p122-RhoGAP
  • rho GTPase-activating protein 7
  • Rho-GTPase-activating protein 7
  • Rho-type GTPase-activating protein 7
  • StARD12
  • StAR-related lipid transfer (START) domain containing 12
  • StAR-related lipid transfer protein 12
  • START domain-containing protein 12

Background

This gene is deleted in the primary tumor of hepatocellular carcinoma. It maps to 8p22-p21.3, a region frequently deleted in solid tumors. It is suggested that this gene is a candidate tumor suppressor gene for human liver cancer, as well as for prostate, lung, colorectal, and breast cancers. Alternative splicing at this locus results in several transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DLC1 Antibody (NB300-917)(2)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DLC1
Entrez
Uniprot
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