PGLYRP2/PGRP-L Antibody (439728) [Unconjugated]

Mouse liver single‑cell suspension was stained with Rat Anti-Mouse PGLYRP2/PGRP-L Monoclonal Antibody (Catalog # MAB4704, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 439728
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

PGLYRP2/PGRP-L Antibody (439728) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse PGLYRP2/PGRP-L
  Ser23-Asn530
  Accession # Q8VCS0
• Specificity: Detects mouse PGLYRP2/PGRP-L in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) PGRP-S and rmPGRP-1 beta is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PGLYRP2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGLYRP2/PGRP-L Antibody (439728) [Unconjugated]

• EC 3.5.1.28
• peptidoglycan recognition protein 2tagl-beta
• peptidoglycan recognition protein L
• PGLYRP2
• PGLYRPL
• PGLYRPLHMFT0141
• PGRP2
• PGRPL
• PGRP-L
• PGRP-LN-acetylmuramoyl-L-alanine amidase
• PGRPLPeptidoglycan recognition protein long
• TAGL
• tagL-alpha
• TAGL-like

Background

PGRP-L (peptidoglycan recognition protein‑long; also called PGRP-2 or TagL) is a 530 amino acid (aa) member of the PGRP family that is expressed in the liver. All mouse PGRPs bind peptidoglycan and Gram-positive bacteria, and may function as innate immunity pattern recognition molecules. PGRP-L is a Zn²⁺-dependent N‑acetylmuramoyl-L-alanine amidase that hydrolyzes cell wall glycopeptides. It contains two membrane-spanning segments, with both termini thought to be extracellular, but is also reported in serum. A truncated 450 aa isoform has been reported. Overall aa identity between mouse and human PGRP-L is 68%.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGLYRP2
• Uniprot:
  • Q8VCS0()