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AMPD1 Antibody

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Western Blot: AMPD1 Antibody [NBP2-24509] - Analysis of human AMPDA1 in mouse skeletal muscle lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat skeletal muscle using NBP2-24509 at 1 ug/ml.
Western Blot: AMPD1 Antibody [NBP2-24509] - Analysis of human AMPDA1 in mouse skeletal muscle lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat skeletal muscle using NBP2-24509 at 1 ug/ml.
Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP2-24509] - IHC analysis of human skeletal muscle using NBP2-24509 at 10 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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AMPD1 Antibody Summary

Immunogen
A portion of amino acids 140-190 of human AMPDA1 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AMPD1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Publications
Read Publication using NBP2-24509.

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a and CRA_c), mouse, rat (isoforms CRA_a and CRA_b), chimpanzee, orangutan, macaque and rhesus monkey and 88% homologous in opossum and dog.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AMPD1 Antibody

  • adenosine monophosphate deaminase 1 (isoform M)
  • adenosine monophosphate deaminase 1
  • adenosine monophosphate deaminase-1 (muscle)
  • AMP deaminase 1
  • AMP deaminase isoform M
  • AMPD
  • EC 3.5.4.6
  • MAD
  • MADA
  • Myoadenylate deaminase
  • skeletal muscle AMPD

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
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Western Blot: Exosome component 10 Antibody [NBP1-32870] - analysis of EXOSC10 in HeLa whole cell extracts treated with (1) si-control and (2) EXOSC10 siRNA using anti-EXOSC10 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Exosome component 10 Antibody [NBP1-32870] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
Western Blot: Exosome component 10 Antibody [NBP1-32870] - Sample: 1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of EXOSC 10 protein by 2. 5 ug of EXOSC 10 antibody 7. 5% SDS-PAGE gel.
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Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.
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Immunohistochemistry: GARS Antibody (4G4) [NBP2-45808] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: GARS Antibody (4G4) [NBP2-45808] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-49690] - Total protein from MCF7 cells, human and mouse kidney were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-PGRP-L in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-49690] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGRP-L at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Western Blot: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.
Western Blot: NCOR1 Antibody [NB100-58824] - Mouse whole cell lysate.
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Western Blot: MARCH8 Antibody [NBP1-85540] - Analysis in control (vector only transfected HEK293T lysate) and LY408083 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: MARCH8 Antibody [NBP1-85540] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
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Applications: WB, IHC, IHC-P
Recombinant Human
MYLIP Protein
H00029116-P01
12.5% SDS-PAGE Stained with Coomassie Blue.
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MAX binding
protein Antibody
NBP2-04053
Immunoprecipitation: MAX binding protein Antibody [NBP2-04053] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MNT antibody NBP2-04052 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. MNT was also immunoprecipitated by rabbit anti-MNT antibody NBP2-04053, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Species: Hu
Applications: WB, IP
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Bioinformatics

Gene Symbol AMPD1