AMPD1 Antibody

Western Blot: AMPD1 Antibody [NBP2-24509] - Analysis of human AMPDA1 in mouse skeletal muscle lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat skeletal muscle using NBP2-24509 at 1 ug/ml.

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP2-24509] - Analysis of human skeletal muscle using NBP2-24509 at 10 ug/ml.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ma-Op, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

AMPD1 Antibody Summary

• Immunogen: A portion of amino acids 140-190 of human AMPDA1 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMPD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml

Reactivity Notes

100% homologous in human (isoforms CRA_a and CRA_c), mouse, rat (isoforms CRA_a and CRA_b).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AMPD1 Antibody

• adenosine monophosphate deaminase 1 (isoform M)
• adenosine monophosphate deaminase 1
• adenosine monophosphate deaminase-1 (muscle)
• AMP deaminase 1
• AMP deaminase isoform M
• AMPD
• EC 3.5.4.6
• MAD
• MADA
• Myoadenylate deaminase
• skeletal muscle AMPD

Background

Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPD1 Antibody (NBP2-24509)(2)

Publications using NBP2-24509

• Yamada T, Ashida Y, Tamai K et al. Improved skeletal muscle fatigue resistance in experimental autoimmune myositis mice following high-intensity interval training Arthritis research & therapy 2022-06-27 [PMID: 35761371] (WB, Mouse)
• Coley W, Rayavarapu S, Pandey GS et al. The molecular basis of skeletal muscle weakness in a mouse model of inflammatory myopathy. Arthritis Rheum. 2012-11-01 [PMID: 22806328]

Bioinformatics

• Gene Symbol: AMPD1