MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">
MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Mouse liver single-cell suspension was stained with Rat Anti-Mouse PGLYRP2/PGRP-L Monoclonal Antibody (Catalog # MAB4704, filled histogram) or isotype control antibody (Catalog #
MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')
2Secondary Antibody (Catalog #
F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Mouse liver single-cell suspension was stained with Rat Anti-Mouse PGLYRP2/PGRP-L Monoclonal Antibody (Catalog # MAB4704, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />
![Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-49690] - Total protein from MCF7 cells, human and mouse kidney were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-PGRP-L in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/PGLYRP2-PGRP-L-Antibody-Western-Blot-NBP2-49690-img0001.jpg "Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-49690] - Total protein from MCF7 cells, human and mouse kidney were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-PGRP-L in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-49690] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGRP-L at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PGLYRP2-PGRP-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49690-img0002.jpg)
![Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-32042] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)](http://images.novusbio.com/fullsize/PGRPL-Antibody-Western-Blot-NBP2-32042-img0002.jpg "Western Blot: PGLYRP2/PGRP-L Antibody [NBP2-32042] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)")
![Immunohistochemistry-Paraffin: PGLYRP2/PGRP-L Antibody [NBP2-32042] - Staining of human liver shows distinct cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PGRPL-Antibody-Immunohistochemistry-NBP2-32042-img0001.jpg)
![Immunohistochemistry-Paraffin: PGLYRP2/PGRP-L Antibody [NBP2-32042] -Staining of human testis shows strong extracellular space positivity in Leydig cells.](http://images.novusbio.com/fullsize/antibody/nbp2-32042_rabbit-polyclonal-pglyrp2-pgrp-l-antibody-immunohistochemistry-paraffin-102202593354..png)
![Immunohistochemistry-Paraffin: PGLYRP2/PGRP-L Antibody [NBP2-32042] -Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-32042_rabbit-polyclonal-pglyrp2-pgrp-l-antibody-immunohistochemistry-paraffin-102202593449..png)
![Immunohistochemistry-Paraffin: PGLYRP2/PGRP-L Antibody [NBP2-32042] -Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-32042_rabbit-polyclonal-pglyrp2-pgrp-l-antibody-immunohistochemistry-paraffin-102202593540..png)
![Immunohistochemistry: PGLYRP2/PGRP-L Antibody [NBP2-31930] - Staining of human liver shows distinct cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PGLYRP2-PGRP-L-Antibody-Immunohistochemistry-NBP2-31930-img0003.jpg "Immunohistochemistry: PGLYRP2/PGRP-L Antibody [NBP2-31930] - Staining of human liver shows distinct cytoplasmic positivity in hepatocytes.")
![Immunocytochemistry/Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-57939] - Staining of human cell line MCF7 shows localization to cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PGLYRP2-PGRP-L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57939-img0001.jpg "Immunocytochemistry/Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-57939] - Staining of human cell line MCF7 shows localization to cell junctions. Antibody staining is shown in green.")
