PGLYRP3/PGRPIA Antibody (187C434)

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Western Blot: PGLYRP3/PGRPIA Antibody (187C434) [NB100-56729] - Analysis in cell lysates from human brain using this antibody at a dilution of 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
187C434
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

PGLYRP3/PGRPIA Antibody (187C434) Summary

Immunogen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 178-193 of human PGRP-1a.
Specificity
PGRP-1a (187C434)
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
PGLYRP3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Application Notes
Flow Cytometry (Cell Surface): see Uehara et al, (2005) Immunocytochemistry/Immunofluorescence: (see Uehara et al, 2005)
Positive Control
Brain Lysate (NB820-59177)
Publications
Read Publications using
NB100-56729 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PGLYRP3/PGRPIA Antibody (187C434)

  • peptidoglycan recognition protein 3
  • peptidoglycan recognition protein I alpha
  • Peptidoglycan recognition protein I-alpha
  • Peptidoglycan recognition protein intermediate alpha
  • peptidoglycan recognition protein-I-alpha
  • PGLYRP3
  • PGLYRPIalphaMGC149197
  • PGRP1A
  • PGRPI alpha
  • PGRP-I alpha
  • PGRPIA
  • PGRP-Ialpha
  • PGRPIAPGRP-I-alpha

Background

Primary immune recognition is based on structures common among invading pathogens. Bacterial surface molecules, such as lipopolysaccharide (LPS) and peptidoglycan (PGN), are known to elicit immune reactions ranging from cytokine release to fever. Recently, a family of proteins called peptidoglycan recognition protein (PGRP) has been identified in mouse and human that binds to peptidoglycans expressed on Gram-positive bacteria. Peptidoglycan (PGN) is an essential cell wall component of virtually all bacteria and, thus, it is an excellent target for recognition by the eukaryotic innate immune system. The PGRPs (PGRP-L, PGRP-S, PGRP-1a (PGRP-3), and PGRP-1beta (PGRP-4)) define a new family of human pattern recognition molecules. PGRP-L is primarily expressed in the liver. Although liver is not considered a primary immune organ, liver participates in host defenses by producing acute phase proteins (by hepatocytes) in response to infections and by clearing microorganisms from blood. PGRP-1a mRNA is highly expressed in esophagus, tonsils and thymus and to lesser extent in the stomach, rectum, and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for PGLYRP3/PGRPIA Antibody (NB100-56729)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow-CS, Flow-IC, ICC/IF.


Filter By Application
Flow-CS
(3)
Flow-IC
(1)
ICC/IF
(3)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-56729 Applications Species
Hua X, Yuan X, Li Z et al. A Novel Innate Response of Human Corneal Epithelium to Heat-killed Candida albicans by Producing Peptidoglycan Recognition Proteins PLoS ONE. 2015 Jun 04 [PMID: 26039076] (ICC/IF, Human)

Details:
PGLYRP3/PGRPIA Antibody (clone 187C434; Imgenex IMG-391) was used for ICC-IF staining on human corneal epithelial cells /HCECs that were incubated in the absence or presence of isotype IgG (10ug/ml), dectin-1 neutralizing Ab (10ug/ml), BAY11-7082 (10uM) or NF-kB activation inhibitor quinazoline (NF-kB-I, 10uM) for 1 h followed by 48 hours incubation of HKCA/heat-killed Candida albicans (10^6 cells/ml). The immunoassay implicated 10 minutes RT fixation with 2% PFA, 10 minutes RT permeabilization with PBS-0.2% Triton X-100, detection of primary using Alexa-Fluor 488 conjugated secondary antibody (Fig 4A).
ICC/IF Human
Ma P, Wang Z, Pflugfelder SC, Li DQ. Toll-like receptors mediate induction of peptidoglycan recognition proteins in human corneal epithelial cells. Exp Eye Res. 2010 Jan [PMID: 19799901] (ICC/IF, Human)

Details:
PGRP-1alpha (IMG-391) & PGRP-1beta (IMG-414). IF/ICC: Human corneoscleral tissue & primary cultured human corneal epithelial cells, Fig 1.
ICC/IF Human
Uehara A, Takada H. Synergism between TLRs and NOD1/2 in oral epithelial cells. J Dent Res. 2008 Jul [PMID: 18573991] (Flow-CS)

Details:
flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), PGRP-S (IMG-393). Oral epithelial HSC-2 cell line stimulated with Fk156 or muramyldipeptide (MDP) plus FSL-1 and lipid A, Fig 1.
Flow-CS
Uehara A, Fujimoto Y, Fukase K, Takada H. Various human epithelial cells express functional Toll-like receptors, NOD1 and NOD2 to produce anti-microbial peptides, but not proinflammatory cytokines. Mol Immunol. 2007 May [PMID: 17403538] (Flow-CS, Human)

Details:
The following products were used in flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), PGRP-S (IMG-393). Human oral epithelial (HSC-2, HSC-3, SAS, & HO-1-u-1), human pharyngeal epithelial (HEp-2), human esophageal epithelial (TE-1), human b
Flow-CS Human
Uehara A, Fujimoto Y, Kawasaki A et al. Meso-diaminopimelic acid and meso-lanthionine, amino acids specific to bacterial peptidoglycans, activate human epithelial cells through NOD1. J Immunol. 2006 Aug 1 [PMID: 16849490] (Flow-IC)

Details:
flow (Intracellular): PGRP-1alpha (IMG-391) & PGRP-1beta (IMG-414); Human oral epithelial (HSC-2) cell line, Fig 2.
Flow-IC
Uehara A, Sugawara Y, Kurata S et al. Chemically synthesized pathogen-associated molecular patterns increase the expression of peptidoglycan recognition proteins via toll-like receptors, NOD1 and NOD2 in human oral epithelial cells. Cell Microbiol. 2005 May [PMID: 15839897] (Flow-CS, ICC/IF)

Details:
Flow (cell surface): PGRP-1alpha (IMG-391), PGRP-1beta (IMG-414), and PGRP-S (IMG-393) were used in human oral epithelial (HSC-2) cell lines, Fig 3. IF/ICC: HSC-2 cells stimulated with or without lipid A, muramyldipeptide (MDP), gama-D-glutamyl-meso-DAP (iE-DAP), or IFN gamma then stained with PGRP-1beta (IMG-414), Fig 4.
Flow-CS, ICC/IF

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Positive Control Lysate(s)

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Bioinformatics

Gene Symbol PGLYRP3
Entrez
Uniprot