PGC-1 beta Antibody - BSA Free

Western Blot: PGC-1 beta Antibody [NBP1-28722] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PGC-1beta antibody NBP1-28722 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. PGC-1beta was also immunoprecipitated by rabbit anti-PGC-1beta antibody NBP1-28721, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Western Blot: PGC-1 beta Antibody [NBP1-28722] - Whole cell lysate (50 ug) from HeLa, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PGC-1beta antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PGC-1 beta Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human peroxisome proliferator-activated receptor gamma coactivator 1 beta using the numbering given in entry NP_573570.2 (GeneID 133522).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARGC1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGC-1 beta Antibody - BSA Free

• DKFZp686C1790
• ERRL1
• PERCFLJ14284
• peroxisome proliferator-activated receptor gamma, coactivator 1 beta
• PGC1
• PGC1Bgamma, coactivator 1, beta
• PPARGC1

Background

Peroxisome proliferator-activated receptor gamma coactivator 1-beta (PGC-1beta) is a member of a family of transcriptional coactivators that play a role in metabolism and whole-body homeostasis. PGC-1beta has been shown to be a co-activator of the estrogen receptor alpha, glucocorticoid receptor, and the nuclear respiratory factor 1 (NRF1). Additionally PGC-1beta is suggested to be integral to mitochondrial function and glucose metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGC-1 beta Antibody (NBP1-28722)(3)

Publications using NBP1-28722

• Stevens DA, Lee Y, Kang HC et al. Parkin loss leads to PARIS-dependent declines in mitochondrial mass and respiration. Proc. Natl. Acad. Sci. U.S.A. 2015-09-15 [PMID: 26324925] (WB, Human)
• Kammerer M, Gutzwiller S, Stauffer D et al. Estrogen Receptor alpha (ERalpha) and Estrogen Related Receptor alpha (ERRalpha) are both transcriptional regulators of the Runx2-I isoform. Mol Cell Endocrinol 2013-02-08 [PMID: 23403054] (WB, Human)
• Liu Y, Zhou D, Zhang F et al. Liver Patt1 deficiency protects male mice from Age-associated but not high-fat diet-induced hepatic steatosis. Journal of Lipid Research. 2012-01-09 [PMID: 22231784]

Review for PGC-1 beta Antibody (NBP1-28722) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Masashi Okada WB Human 09/24/2023

Summary

• Application: Western Blot
• Sample Tested: Human Glioma cells, whole cell lysates
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: PPARGC1B
• Entrez:
  • 133522(Human)
• Uniprot:
  • Q86YN6()