Periostin/OSF-2 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

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Periostin/OSF-2 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Periostin/OSF-2 (R&D Systems, Catalog # 3548-F2)
Ala22-Gln836
Accession # Q15063
Specificity
Detects human Periostin/OSF-2 in Western blots. In this format, approximately 45% cross-reactivity with recombinant mouse Periostin/OSF-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POSTN
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Periostin/OSF-2 Antibody [Biotin]

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Human OSF-2 (osteoblast-specific factor 2; also periostin) is a 170 kDa secreted homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules (1-4). It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor (2, 5, 6, 7). It is synthesized as an 836 amino acid (aa) precursor that contains a 21 aa signal sequence and an 815 aa mature region (2, 8). It is unknown if the molecule has any significant glycosylation (2). The human homodimer is not disulfide-linked (3). The molecule consists of two distinct regions. The N-terminus contains an 55 aa EMI domain, while the C-terminus contains four 130 aa fasciculin type 1 (FAS1) domains. The EMI domain is cysteine-rich and shows a highly basic alpha -helix (9). Each FAS1 repeat exhibits a novel seven-stranded beta ‑wedge with a multiple alpha -helical fold (1, 8). Three alternate splice forms are known that are C-terminal to the fourfold FAS1 repeats. These mature molecules are 758 and 761 aa in length. The first shows a one aa substitution for aa 649-706 of the mature molecule. The second shows a one aa substitution for aa 649-676, and a deletion of 27 aa between aa 784-810 of the mature molecule (10). The significance of the alternate splice forms is not clear. OSF-2 is known to bind to alpha v beta 3 and alpha v beta 5 integrins (3). It is synthesized by smooth muscle cells, fibroblasts, osteoblasts, and multiple carcinoma cell types (2, 3, 5, 7, 11, 12). OSF-2 induces expression of VEGFR2/KDR on endothelial cells (EC) by binding to EC alpha v beta 3 (12). It also promotes cell transformation to a tumorigenic phenotype, accompanied by MMP-9 and fibronectin production and cell migration (13). Mature human OSF-2 is 91%, 96% and 91% aa identical to rat, dog, and mouse OSF-2, respectively.

  1. Clout, N.J. and D. Tisi (2003) Structure 11:197.
  2. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  3. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  4. Litvin, J. et al. (2005) Anat. Rec. A Discov. Mol. Cell. Evol. Biol. 287A:1205.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  7. Yoshioka, N. et al. (2002) Exp. Cell Res. 279:91.
  8. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  9. Callebaut, I. et al. (2003) Biochem. Biophys. Res. Commun. 300:619.
  10. Swiss-Prot Accession # Q15063.
  11. Li, G. et al. (2006) Atherosclerosis 188:292.
  12. Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
  13. Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Biotin BAF3548

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Bioinformatics

Gene Symbol POSTN
Uniprot