Perilipin-2/ADFP Antibody (788620)

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and JAR human choriocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-2 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Perilipin-2 at approximately 58 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB7634). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

ApoE2 and apoE3, but not apoE4, partially rescue alpha -synuclein accumulation in apoE-deficient cerebral organoids. The APOE−/− iPSC-derived cerebral organoids at Day 90 were treated with conditioned media of immortalized astrocytes from APOE2-target replacement (TR), APOE3-TR, or APOE4-TR mice for 5 days. Conditioned media from Apoe-KO astrocytes were used as a control. a, A schematic workflow of the rescue experiments. b–i, Amounts of EEA1 (c), LAMP1 (d), GCase (e), LIMP1 (f), Plin2 (g), alpha Syn (h) and p-alpha Syn (i) in the RIPA fraction of the cerebral organoids after treatments were quantified by Western blotting. j–l, Amounts of total alpha Syn (k) and p-alpha Syn (l) in the SDS fraction of cerebral organoids after treatments were quantified by Western blotting. All data were normalized to beta -actin levels. 3 cerebral organoids were pooled and analyzed as one sample. All data are expressed as mean ± SEM (n = 5 samples/each). Experiments were repeated in three independent differentiation batches. One-way ANOVA was performed to determine statistical significance. **p < 0.01, ****p < 0.0001, n.s., not significant Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34453582), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 788620
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Perilipin-2/ADFP Antibody (788620) Summary

• Immunogen: E. coli-derived recombinant human Perilipin-2
  Met1-His437
  Accession # Q99541
• Specificity: Detects human Perilipin-2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLIN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 4 ug/mL
  • Western Blot 0.2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Perilipin-2/ADFP Antibody (788620)

• ADFP
• Adipophilin
• Adipose differentiation-related proteinadipophilin
• ADRP
• MGC10598
• perilipin 2
• Perilipin2
• Perilipin-2
• PLIN2

Background

Perilipin-2 (PLIN2), also known as Adipophilin (ADFP) and Adipose Differentiation-Related Protein (ADRP), is a widely expressed 52 kDa acylated protein that associates with membranes and cytoplasmic lipid droplets. It is upregulated during adipocyte differentiation and is a major component of milk fat globules. Perilipin-2 promotes the cellular uptake and accumulation of long chain fatty acids and regulates the intracellular distribution of phospholipids. It promotes hepatic insulin resistance in response to a high fat diet. Human Perilipin-2 shares 82% and 79% aa sequence identity with mouse and rat Perilipin-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Perilipin-2/ADFP Antibody (MAB7634)(3)

Publications using MAB7634

• Kate M Warde, Yi Jan Lim, Eduardo Ribes Martinez, Felix Beuschlein, Paula O’Shea, Constanze Hantel et al. Mitotane Targets Lipid Droplets to Induce Lipolysis in Adrenocortical Carcinoma Endocrinology 2022-09-01 [PMID: 35797592]
• Ioanna Korovila, Annika Höhn, Tobias Jung, Tilman Grune, Christiane Ott Reduced Liver Autophagy in High-Fat Diet Induced Liver Steatosis in New Zealand Obese Mice Antioxidants (Basel) 2021-03-24 [PMID: 33804819]
• Linda L. Lee, Hnin H. Aung, Dennis W. Wilson, Steven E. Anderson, John C. Rutledge, Jennifer M. Rutkowsky Triglyceride-rich lipoprotein lipolysis products increase blood-brain barrier transfer coefficient and induce astrocyte lipid droplets and cell stress American Journal of Physiology-Cell Physiology 2017-04-01 [PMID: 28077357]

Bioinformatics

• Gene Symbol: PLIN2
• Uniprot:
  • Q99541(Human)