PDX-1/IPF1 Antibody

Images

 
Western Blot: PDX1 Antibody [NBP2-22150] - WB analysis of PDX1 in INS1 cell lysate.
Immunocytochemistry/ Immunofluorescence: PDX1 Antibody [NBP2-22150] - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: PDX1 Antibody [NBP2-22150] - IHC analysis of PDX1 in mouse pancreas.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

PDX-1/IPF1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human PDX1 protein (between residues 100-200) [UniProt P52945]
Localization
Nucleus, cytoplasm.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This PDX1 antibody is useful for Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. In Western blot a band is observed ~40 kDa, and in ICC/IF diffuse nuclear and cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Human and mouse. Predicted to react with rat based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDX-1/IPF1 Antibody

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

PDX1, also known as IDX-1, IPF-1, STF-1, and MODY4 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. During development, PDX1 specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, it is required for maintaining the hormone-producing phenotype of the beta-cell. PDX1 is located in both the nucleus and cytosol on a cellular level, while in tissue it is distinct to the duodenum and pancreas. Defects in PDX1 gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). Recently, research of PDX1 ranges from an important role in the malaria parasite (PMID: 23039077) to mutations in PDX1 leading to neonatal diabetes (PMID: 20009086).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PDX1
Entrez
Uniprot