PDX-1/IPF1 Antibody - BSA Free

Western Blot: PDX1 Antibody [NBP2-22150] - WB analysis of PDX1 in INS1 cell lysate.

Immunocytochemistry/Immunofluorescence: PDX1 Antibody [NBP2-22150] - PDX1 antibody was tested in INS1 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: PDX1 Antibody [NBP2-22150] - IHC analysis of PDX1 in mouse pancreas.

• Reactivity: Hu, Mu, Ca, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PDX-1/IPF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PDX-1/IPF1 Antibody - BSA Free (NBP2-22150) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PDX-1/IPF1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to a C-terminal portion of the human PDX1 protein (between residues 100-200) [UniProt P52945]
• Localization: Nucleus, cytoplasm.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1.0 ug/ml
• Application Notes: In Western blot a band is observed ~40 kDa, and in ICC/IF diffuse nuclear and cytoplasmic staining is observed. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Predicted to react with rat based on 100% sequence homology. Canine reactivity reported in scientific literature (PMID: 30332726).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDX-1/IPF1 Antibody - BSA Free

• Glucose-sensitive factor
• IDX1
• IDX-1GSF
• Insulin promoter factor 1
• insulin promoter factor 1, homeodomain transcription factor
• Insulin upstream factor 1
• IPF1
• IPF1pancreas/duodenum homeobox protein 1
• Islet/duodenum homeobox-1
• MODY4IUF1
• pancreatic and duodenal homeobox 1
• pancreatic-duodenal homeobox factor 1
• PDX-1
• PDX-1IPF-1
• somatostatin transcription factor 1
• Somatostatin-transactivating factor 1
• STF-1IUF-1

Background

PDX1, also known as IDX-1, IPF-1, STF-1, and MODY4 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. During development, PDX1 specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, it is required for maintaining the hormone-producing phenotype of the beta-cell. PDX1 is located in both the nucleus and cytosol on a cellular level, while in tissue it is distinct to the duodenum and pancreas. Defects in PDX1 gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). Recently, research of PDX1 ranges from an important role in the malaria parasite (PMID: 23039077) to mutations in PDX1 leading to neonatal diabetes (PMID: 20009086).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDX-1/IPF1 Antibody (NBP2-22150)(2)

Publications using NBP2-22150

• Aguiar BA, Orechio D, Fratini P et al. Isolation and Characterization of Pancreatic Canine Fetal Cells at the Final Stage of Gestation. Anat Rec (Hoboken). 2018-10-17 [PMID: 30332726] (Canine)
• Ramdas M, Sharma S, Kaul D, Bhatia A. Possible role of miR-2909 RNomics in arsenic mediated pancreatic B-cell dysfunction. Journal of Trace Elements in Medicine and Biology 2018-12-01 [PMID: 30262289] (WB, Mouse)

Bioinformatics

• Gene Symbol: PDX1
• Entrez:
  • 3651(Human)
  • 18609(Mouse)
• Uniprot:
  • P52945()
  • P52946()