PDK4 Antibody

Images

 
Western Blot: PDK4 Antibody [NBP1-07049] - Western Blot on human heart protein lysate.
Immunocytochemistry/ Immunofluorescence: PDK4 Antibody [NBP1-07049] - PDK4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.24 mg/ml

PDK4 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human PDK4 protein (within residues 200-250). [Swiss-Prot# Q16654]
Localization
Mitochondrion matrix.
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This PDK4 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~49 kDa. In ICC/IF mitochondrial staining was observed in HeLa cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PDK4 Lysate (NBL1-14252)
Heart Lysate (NB820-59217)
Control Peptide
PDK4 Peptide (NBP1-07049PEP)
Publications
Read Publication using
NBP1-07049 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human. Immunogen sequence has 94% homology with rat and 88% homology with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.24 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDK4 Antibody

  • [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial
  • EC 2.7.11
  • EC 2.7.11.2
  • FLJ40832
  • Pyruvate dehydrogenase kinase isoform 4
  • pyruvate dehydrogenase kinase, isoenzyme 4
  • pyruvate dehydrogenase kinase, isozyme 4
  • pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial

Background

PDK4 (pyruvate dehydrogenase kinase isoform 4) is expressed ubiquitously in the mitochondrion matrix of tissues with highest expression seen in liver, heart and skeletal muscles. PDK4 is regulated by glucocorticoids, retinoic acid and insulin, and is found elevated with diabetes, fasting and other conditions where fatty acids act as energy source. Under starvation/diabetic conditions, enhanced glucocorticoids levels activate glucocorticoid receptor thereby recruiting p300/CBP coactivator complex to PDK4 gene where it cooperatively interact with FOXO factors and activated RARs to make a stable transcriptional initiation complex responsible for PDK4 activation. Insulin promotes inactivation and translocation of FOXO factors from nucleus to cytoplasm and thereby suppresses glucocorticoid-stimulated PDK4 gene transcription. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex (PDC) via its E1 alpha subunit's phosphorylation, thus contributing to glucose metabolism regulation. Insulin resistance is associated with PDC dysregulation in skeletal muscles and excessive insulin on the other hand downregulates PDK4 expression. PDK4 exerts conservation of glucose as well as three-carbon compounds such as lactate, alanine, pyruvate etc. which serve as substrates for gluconeogenesis and this conservation helps maintain euglycemia during starvation, however, exacerbates hyperglycemia in diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDK4 Antibody (NBP1-07049)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

View specific protocols for PDK4 Antibody (NBP1-07049): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDK4 Antibody (NBP1-07049). (Showing 1 - 1 of 1 FAQ).

  1. Which PDK4 antibody do you recommend for immunoblot? I'm working with human cell lines.
    • NBP1-07047 and NBP1-07049 are our best selling PDK4 antibodies that will meet your mentioned criteria i.e. Immunoblot/Western Blot in Human samples. However, all of our products are 100% guaranteed and you may find a complete list of PDK4 antibodies by searching PDK4 on our website and narrowing your results using the filters on the left-hand side of the screen.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-07049AF405
Alexa Fluor 488 Labeled NBP1-07049AF488
Alexa Fluor 647 Labeled NBP1-07049AF647
Alexa Fluor 700 Labeled NBP1-07049AF700
Biotin Labeled NBP1-07049B
DyLight 405 Labeled NBP1-07049V
DyLight 488 Labeled NBP1-07049G
DyLight 550 Labeled NBP1-07049R
DyLight 650 Labeled NBP1-07049C
FITC Labeled NBP1-07049F
HRP Labeled NBP1-07049H

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Bioinformatics

Gene Symbol PDK4
Entrez
Uniprot