Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody

Images

 
Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).
Immunocytochemistry/ Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.42 mg/ml

Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]
Localization
Mitochondrion
Predicted Species
Mouse (100%), Rat (100%), Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
Application Notes
This PDK1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Pyruvate Dehydrogenase Kinase 1/PDK1 Overexpression Lysate
Human Heart Whole Tissue Lysate (Adult Whole Normal)
Pyruvate Dehydrogenase Kinase 1/PDK1 Knockout HeLa Cell Lysate
Control Peptide
Pyruvate Dehydrogenase Kinase 1/PDK1 Protein (NB100-2383PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-2383 in the following applications:

Publications
Read Publications using
NB100-2383 in the following applications:

Reactivity Notes

Predicted to react with mouse and rat based on 100% sequence homology. Immunogen displays the following percentage of sequence identity for non-tested species: chicken (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.42 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody

  • EC 2.7.11
  • EC 2.7.11.2
  • mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
  • PDH kinase 1
  • PDK1
  • PDK-1
  • PKB kinase
  • Pyruvate dehydrogenase kinase isoform 1
  • pyruvate dehydrogenase kinase, isoenzyme 1
  • pyruvate dehydrogenase kinase, isozyme 1
  • Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial

Background

Pyruvate dehydrogenase kinase isoform 1 (PDK1) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2383)(5)

Reviews for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2383) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-2383:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Human 08/22/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol
Western Blot Pyruvate Dehydrogenase Kinase 1/PDK1 NB100-2383
Enlarge
5
reviewed by:
Anonymous
WB Human 01/26/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates, Sample Amount: 50
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% milk, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1/1000, Incubation Dilution Buffer: 5 % milk, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Donkey anti-rabbit IgG-HRP

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 49 kDa

Product General Protocols

View specific protocols for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2383): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2383) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2383AF350
Alexa Fluor 405 NB100-2383AF405
Alexa Fluor 488 NB100-2383AF488
Alexa Fluor 532 NB100-2383AF532
Alexa Fluor 594 NB100-2383AF594
Alexa Fluor 647 NB100-2383AF647
Alexa Fluor 700 NB100-2383AF700
Alexa Fluor 750 NB100-2383AF750
Biotin NB100-2383B
DyLight 350 NB100-2383UV
DyLight 405 NB100-2383V
DyLight 488 NB100-2383G
DyLight 550 NB100-2383R
DyLight 594 NB100-2383DL594
DyLight 650 NB100-2383C
DyLight 680 NB100-2383FR
DyLight 755 NB100-2383IR
FITC NB100-2383F
HRP NB100-2383H
Janelia Fluor 549 NB100-2383JF549
Janelia Fluor 646 NB100-2383JF646

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
08/22/2015
Application: WB
Species: Human

Anonymous
01/26/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol PDK1