Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody

Western Blot: PDK-1 Antibody [NB100-2383] - Analysis of HeLa lysates using NB100-2383. Image courtesy of Gregg Semenza - publication number 21620138 (PMID).

Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [NB100-2383] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Western Blot: PDK-1 Antibody [NB100-2383] - Detection of PDK-1 in human heart lysate using NB 100-2383 at 2 ug/ml.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]
• Localization: Mitochondrion
• Predicted Species: Mouse (100%), Rat (100%), Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
  • Western Blot 2 ug/ml
• Application Notes: By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Pyruvate Dehydrogenase Kinase 1/PDK1 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Pyruvate Dehydrogenase Kinase 1/PDK1 Protein (NB100-2383PEP)

Reactivity Notes

Predicted to react with mouse and rat based on 100% sequence homology. Immunogen displays the following percentage of sequence identity for non-tested species: chicken (93%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody

• EC 2.7.11
• EC 2.7.11.2
• mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
• PDH kinase 1
• PDK1
• PDK-1
• PKB kinase
• Pyruvate dehydrogenase kinase isoform 1
• pyruvate dehydrogenase kinase, isoenzyme 1
• pyruvate dehydrogenase kinase, isozyme 1
• Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial

Background

Pyruvate dehydrogenase kinase isoform 1 (PDK1) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2383)(5)

Publications using NB100-2383

• Dey P, Kundu A et al. PKM2 Knockdown Induces Autophagic Cell Death via AKT/mTOR Pathway in Human Prostate Cancer Cells. Cell Physiol Biochem 2019-05-29 [PMID: 31135122] (WB, Human)
• Sung WW, Chen PR, Liao MH, Lee JW. Enhanced aerobic glycolysis of nasopharyngeal carcinoma cells by Epstein-Barr virus latent membrane protein 1 Exp. Cell Res. 2017-08-04 [PMID: 28827059] (WB, Human)
• Tsoi H, Li L, Chen ZS et al. The SARS-Coronavirus Membrane protein induces apoptosis via interfering PDK1-PKB/Akt signaling. Biochem. J. 2014-10-01 [PMID: 25271362] (ICC/IF, Human)
  
  Details:
  PDK-1 peptide NB100-2383PEP used for blocking PDK-1 antibody NB100-2383 in ICC-IF staining assay of HEK (human embryonic kidney)-293FT cells transfected or not with M protein (Figure 2 A).
• Luo W, Chang R, Zhong J, Pandey A, Semenza GL. Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression. Proc Natl Acad Sci U S A. 2012-12-04 [PMID: 23129632]
• Luo W, Hu H, Chang R et al. Pyruvate Kinase M2 is a PHD3-Stimulated Coactivator for Hypoxia-Inducible Factor 1. Cell;145(5):732-44. 2011-05-27 [PMID: 21620138]

Reviews for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2383) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Haiquan Lu WB Human 08/22/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

reviewed by: System Admin WB Human 01/26/2010

Summary

• Application: Western Blot
• Sample Tested: HeLa cell lysates, Sample Amount: 50
• Species: Human

Bioinformatics

• Gene Symbol: PDK1