CPT1A Antibody

Images

 
Western Blot: CPT1A Antibody [NB100-53791] - (0.1ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (4 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CPT1A Antibody [NB100-53791] - (4 ug/ml) staining of paraffin embedded Human Kidney Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CPT1A Antibody Summary

Immunogen
Peptide with sequence C-DPAQTVEQRLKLFK corresponding to internal region according to NP_001867.2, NP_001027017.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CPT1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 80 kDa band observed in human liver lysates (calculated MW of 86.2 kDa band according to NP_001027017.1). IHC-P: Human kidney shows mitochindrial staining in PCT.
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59232)
CPT1A Lysate (NBL1-09449)
CPT1A Lysate (NBP2-65666)
Control Peptide
CPT1A Protein (NB100-53791PEP)
Publications
Read Publications using
NB100-53791 in the following applications:

  • WB
    3 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25026599).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CPT1A Antibody

  • carnitine O-palmitoyltransferase 1, liver isoform
  • Carnitine O-palmitoyltransferase I, liver isoform
  • carnitine palmitoyltransferase 1A (liver)
  • Carnitine palmitoyltransferase 1A
  • carnitine palmitoyltransferase I, liver
  • CPT I
  • CPT1
  • CPT1-LEC 2.3.1.21
  • CPTI-L
  • EC 2.3.1
  • L-CPT1

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CPT1A Antibody (NB100-53791)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CPT1A
Entrez
Uniprot