CPT1A Antibody

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Western Blot: CPT1A Antibody [NB100-53791] - Staining of MCF7 cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CPT1A Antibody Summary

Immunogen
Peptide with sequence C-DPAQTVEQRLKLFK corresponding to internal region according to NP_001867.2, NP_001027017.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CPT1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/mL
  • Peptide ELISA Detection limit 1:128000
Application Notes
Western blot: Approx 80-85 kDa band observed in lysates of cell line MCF7 (calculated MW of 86.2 kDa according to NP_001027017.1).
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
CPT1A Overexpression Lysate
CPT1A Knockout 293T Cell Lysate
Control Peptide
CPT1A Protein (NB100-53791PEP)
Publications
Read Publications using
NB100-53791 in the following applications:

  • WB
    6 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25026599). Rat reactivity reported in scientific literature (PMID: 23045433).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CPT1A Antibody

  • carnitine O-palmitoyltransferase 1, liver isoform
  • Carnitine O-palmitoyltransferase I, liver isoform
  • carnitine palmitoyltransferase 1A (liver)
  • Carnitine palmitoyltransferase 1A
  • carnitine palmitoyltransferase I, liver
  • CPT I
  • CPT1
  • CPT1-LEC 2.3.1.21
  • CPTI-L
  • EC 2.3.1
  • L-CPT1

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CPT1A Antibody (NB100-53791)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(6)
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Human
(1)
Mouse
(4)
Rat
(1)
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Showing Publications 1 - 8 of 8.
Publications using NB100-53791 Applications Species
Yang Q, Sun S, Liu W et al. Hypoxia training improves hepatic steatosis partly by downregulation of CB1 receptor in obese mice Biochemical and Biophysical Research Communications Feb 1 2020 [PMID: 32122652] (WB, Mouse) WB Mouse
Zhao L, Niu J, Lin H et al. Paracrine-endocrine FGF chimeras as potent therapeutics for metabolic diseases EBioMedicine 2019 Oct 17 [PMID: 31631034] (WB, Mouse) WB Mouse
Morkholt AS, Trabjerg MS, Oklinski MKE et al. CPT1A plays a key role in the development and treatment of multiple sclerosis and experimental autoimmune encephalomyelitis Sci Rep Sep 16 2019 [PMID: 31527712] (WB, Mouse) WB Mouse
Zhang ZF, Fan SH, Zheng YL et al. Troxerutin improves hepatic lipid homeostasis by restoring NAD(+)-depletion-mediated dysfunction of lipin 1 signaling in high-fat diet-treated mice. Biochem. Pharmacol. 2014 Sep 01 [PMID: 25026599] (WB, Mouse)

Details:
CPT1A/carnitine palmitoyltransferase 1A antibody used for WB on lysates of liver tissues from mouse fed or not with high fat diet and/or treated with Troxerutin (PubChem CID 9896814) or EX527 (PubChem CID 5113032)- loading amount of lysate 50 ug, membrane blocking with 5% non-fat milk in 0.1% Tween-20/TBS, ON 4C primary incubation (Fig 8A).
WB Mouse
Goedeke L, Vales-Lara FM, Fenstermaker M et al. A Regulatory Role for MicroRNA 33(Asterisk) in Controlling Lipid Metabolism Gene Expression. Mol Cell Biol 2013 Jun [PMID: 23547260] (WB, Human) WB Human
Jin K, Norris K, Vaziri ND. Dysregulation of hepatic fatty acid metabolism in chronic kidney disease Nephrol Dial Transplant 2012 Oct 8 [PMID: 23045433] (WB, Rat) WB Rat
Rayner KJ, Esau CC, Hussain FN et al. Inhibition of miR-33a/b in non-human primates raises plasma HDL and lowers VLDL triglycerides. Nature;478(7369):404-407. 2011 Oct 20. [PMID: 22012398]
Pocai A, Lam TK, Obici S et al. Restoration of hypothalamic lipid sensing normalizes energy and glucose homeostasis in overfed rats. J Clin Invest 2006 Apr [PMID: 16528412]

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CPT1A
Entrez
Uniprot