PDGF-BB Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

PDGF-BB Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human PDGF‑BB (R&D Systems, Catalog # 220-BB)
  Ser82-Thr190
  Accession # Q6FHE7
• Specificity: Detects human PDGF-BB in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat PDGF-BB and porcine PDGF is observed, less than 2% cross-reactivity with recombinant human (rh) PDGF-AB is observed, and less than 0.8% cross-reactivity with rhPDGF-AA is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDGFB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR/CF) ELISA Detection: Human PDGF-BB Biotinylated Antibody (Catalog # BAF220) Standard: Recombinant Human PDGF-BB (Catalog # 220-BB)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF-BB Antibody [Biotin]

• c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV
• PDGFBB
• PDGF-BB

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta . Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF-BB Antibody (BAF220)(4)

Publications using BAF220

• N Kanaji, M Yokohira, N Watanabe, N Kadowaki, S Bandoh Transforming Growth Factor-Beta Produced by Non-small Cell Lung Cancer Cells Contributes to Lung Fibroblast Contractile Phenotype Anticancer Res., 2018-04-01;38(4):2007-2014. 4/1/2018 [PMID: 29599317]
• Vascular endothelial growth factor signals through platelet-derived growth factor receptor beta in meningiomas in vitro. Br. J. Cancer, 2012-10-09;107(10):1702-13. 2012-10-09 [PMID: 23047550] (TaqMan Protein Assay, Human)
• John J. Powers, Jason A. Dubovsky, P. K. Epling-Burnette, Lynn Moscinski, Ling Zhang, Satu Mustjoki et al. A molecular and functional analysis of large granular lymphocyte expansions in patients with chronic myelogenous leukemia treated with tyrosine kinase inhibitors Leukemia & Lymphoma 2011-04-01 [PMID: 21271862]
• Lacoste E, Martineau I, Gagnon G Platelet concentrates: effects of calcium and thrombin on endothelial cell proliferation and growth factor release. J. Periodontol., 2003-10-01;74(10):1498-507. 2003-10-01 [PMID: 14653397] (ELISA Development, Human)

Review for PDGF-BB Antibody (BAF220) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human PDGF-BB Biotinylated Affinity.

reviewed by: Steven Moore Flow Human PDGF-BB Biotinylated Affinity 10/13/2020

Summary

• Application: Flow Cytometry
• Sample Tested: Purified PAb
• Species: Human PDGF-BB Biotinylated Affinity
• Lot: Human PDGF-BB Biotinylated AJH1416031

Comments

• Comments: Purified PA b, 50 UG f cyclic AMP micro domains in cardiac myocytes

Bioinformatics

• Gene Symbol: PDGFB
• Uniprot:
  • Q6FHE7()