PDGF-B Antibody (JE75-16)

Western Blot: PDGF-B Antibody (JE75-16) [NBP3-32730] - Western blot analysis of PDGF-B on different lysates with Rabbit anti-PDGF-B antibody (NBP3-32730) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32730) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PDGF-B Antibody (JE75-16) [NBP3-32730] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PDGF-B antibody (NBP3-32730) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32730) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: JE75-16
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

PDGF-B Antibody (JE75-16) Summary

• Description: Novus Biologicals Rabbit PDGF-B Antibody (JE75-16) (NBP3-32730) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human PDGF-B aa 192-241 / 241. (Uniprot: P01127)
• Localization: Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PDGFB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:2000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PDGF-B Antibody (JE75-16)

• B chain
• Becaplermin
• C-Sis
• FLJ12858
• IBGC5
• PDGF subunit B
• PDGF2
• PDGF-2
• PDGFB
• PDGF-B
• platelet-derived growth factor 2
• platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog)
• Platelet-derived growth factor beta polypeptide
• platelet-derived growth factor subunit B
• platelet-derived growth factor, B chain
• Platelet-derived growth factor, beta polypeptide (oncogene SIS)
• Proto-oncogene c-Sis
• SIS
• SSV

Background

Platelet-Derived Growth Factor-BB (PDGF-BB) is a disulfide-linked homodimer of two B-chains (109 aa each). It is stored in platelet granules and is released folllowing activation of platelets. PDGF-BB has potent mitogenic effects on various cell types such as cells of connective tissue, bone and cartilage and smooth muscle. It promotes bone formation and soft tissue repair, stimulating wound healing processes. PDGF-BB is involved in the development of the nervous system and may act as a neuronal regulatory agent via nerve regeneration and proliferation. It also plays a role in the epithelial cell development of the respiratory system, cell migration and angiogenesis. Benefits of NovActive Proteins, Fully Active, Fully folded naturally by eukaryotic source. Comparable for eukaryotic glycosylation BioRisk Free, animal-free, serum-free, endotoxin-free, infection reagent-free, low in proteolyic activity, low in pyrogenic and pro-inflammatory activity. Just reconstitute and add to your cell culture. Have FDA G.R.A.S. (generally recognized as safe) approval. Advantages of barley endosperm, Folding, Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment, A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status. Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDGFB