PDGF-B Antibody

Immunocytochemistry/Immunofluorescence: PDGF-B Antibody [NBP1-52533] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533] - Analysis of anti-PDGFB antibody with human pancreas at concentration 3.75 ug/ml.

Flow Cytometry: PDGF-B Antibody [NBP1-52533] - Flow cytometric analysis of fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: PDGF-B Antibody [NBP1-52533] - Analysis of anti-PDGFB antibody with human placenta at concentration 3.75 ug/ml.

• Reactivity: Hu, Bv, Ca, Sh, Mu, Rt, Bt, Eq, Pm, Ma-Op, Pm
• Applications: ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PDGF-B Antibody Summary

• Immunogen: Peptide with sequence C-KHRKFKHTHDKT, from the C Terminus of the PDGF-B protein sequence according to NP_002599.1 and NP_148937.1.
• Epitope: C-Terminus
• Predicted Species: Mouse (92%), Rat (92%), Bat (92%), Monkey (100%), Primate (92%), Equine (92%), Opossum (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDGFB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA 1:128000
• Application Notes: .

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 28883003).Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (92%), Feline (83%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for PDGF-B Antibody

• B chain
• Becaplermin
• C-Sis
• FLJ12858
• IBGC5
• PDGF subunit B
• PDGF2
• PDGF-2
• PDGFB
• PDGF-B
• platelet-derived growth factor 2
• platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog)
• Platelet-derived growth factor beta polypeptide
• platelet-derived growth factor subunit B
• platelet-derived growth factor, B chain
• Platelet-derived growth factor, beta polypeptide (oncogene SIS)
• Proto-oncogene c-Sis
• SIS
• SSV

Background

Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimmer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF-B Antibody (NBP1-52533)(3)

Publications using NBP1-52533

• Payne LB, Abdelazim H, Hoque M et al. A Soluble Platelet-Derived Growth Factor Receptor-? Originates via Pre-mRNA Splicing in the Healthy Brain and Is Upregulated during Hypoxia and Aging Biomolecules 2023-04-21 [PMID: 37189457] (Canine)
• Payne LB, Abdelazim H, Hoque M et al. A Soluble Platelet-Derived Growth Factor Receptor-? Originates via Pre-mRNA Splicing in the Healthy Brain and is Differentially Regulated during Hypoxia and Aging bioRxiv : the preprint server for biology 2023-02-04 [PMID: 36778261] (ChIP, Mouse)
• Ulve R, Rault M, Bahin M et al. Discovery of human-similar gene fusions in canine cancers Cancer Res. 2017-09-07 [PMID: 28883003] (Canine)

Bioinformatics

• Gene Symbol: PDGFB
• Entrez:
  • 5155(Human)
• Uniprot:
  • P01127()