PBEF/Visfatin/NAMPT Antibody

Images

 
Western blot shows lysates of H4‑II‑E‑C3 rat hepatoma cell line and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat PBEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6384) ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

PBEF/Visfatin/NAMPT Antibody Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21derived recombinant rat PBEF/Visfatin
Gln25-His491
Accession # Q80Z29
Specificity
Detects rat PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse PBEF and recombinant human PBEF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NAMPT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PBEF/Visfatin/NAMPT Antibody

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Background

PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase/Nampt) is a 52‑54 kDa member of the NAPRTase family of molecules. It is a dimer that is found both intracellularly and extracellularly, where it participates in NAD synthesis and induces proinflammatory cytokine synthesis, respectively. PBEF has also been reported to show insulin-like effects on adipocytes by binding to the insulin receptor. Cells known to express PBEF include visceral adipocytes, neutrophils, and capillary endothelium. Rat PBEF is 491 amino acids in length. It contains no signal sequence but is secreted. Potential phosphorylation sites exist at Tyr34, Tyr188 and Ser398. Full‑length (aa 1‑491) rat PBEF shares 99% and 95% aa identity with mouse and human PBEF, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
7270-IL/CF
Species: Hu
NBP1-51641
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC, IHC-FrFl
NBP2-22106
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NBP2-12793
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-31176
Species: Hu, Mu, Sh
Applications: WB, ELISA, IHC, IHC-P
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
AF5739
Species: Hu, Mu, Rt
Applications: WB
NB100-56749
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P

Publications for PBEF/Visfatin/NAMPT Antibody (AF6384) (0)

There are no publications for PBEF/Visfatin/NAMPT Antibody (AF6384).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PBEF/Visfatin/NAMPT Antibody (AF6384) (0)

There are no reviews for PBEF/Visfatin/NAMPT Antibody (AF6384). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PBEF/Visfatin/NAMPT Antibody (AF6384) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional PBEF/Visfatin/NAMPT Products

Bioinformatics Tool for PBEF/Visfatin/NAMPT Antibody (AF6384)

Discover related pathways, diseases and genes to PBEF/Visfatin/NAMPT Antibody (AF6384). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PBEF/Visfatin/NAMPT Antibody (AF6384)

Discover more about diseases related to PBEF/Visfatin/NAMPT Antibody (AF6384).
 

Pathways for PBEF/Visfatin/NAMPT Antibody (AF6384)

View related products by pathway.

PTMs for PBEF/Visfatin/NAMPT Antibody (AF6384)

Learn more about PTMs related to PBEF/Visfatin/NAMPT Antibody (AF6384).

Blogs on PBEF/Visfatin/NAMPT

There are no specific blogs for PBEF/Visfatin/NAMPT, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PBEF/Visfatin/NAMPT Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol NAMPT
Uniprot
COVID-19 update