Pax6 Antibody

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Western Blot: Pax6 Antibody [NB100-61654] - Staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Product Details

Summary
Reactivity Mu, Hu, RtSpecies Glossary
Applications WB, PEP-ELISA, IHC-Fr (-)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Pax6 Antibody Summary

Immunogen
Peptide with sequence C-REEKLRNQRRQASN corresponding to internal region according to NP_001595.2.
Epitope
REEKLRNQRRQASN
Marker
Neural Marker
Specificity
This antibody is expected to recognise both reported isoforms (NP_000271.1 and NP_001595.2).
Predicted Species
Human (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in mouse eye lysates (calculated MW of 48.2 kDa band according to human NP_001595.2, and to mouse NP_038655.1).
Publications
Read Publication using NB100-61654.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Pax6 Antibody

  • keratitis)
  • MGC17209
  • Oculorhombin
  • paired box 6
  • paired box protein Pax-6
  • Pax6

Background

PAX6 is a transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. PAX6 is required for the differentiation of pancreatic islet alpha cells and competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. The Pax6-5A isoform appears to function as a molecular switch that specifies target genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pax6 Antibody (NB100-61654)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Pax6 Antibody (NB100-61654). (Showing 1 - 1 of 1 FAQ).

  1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
    • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

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Blogs on Pax6.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

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Bioinformatics

Gene Symbol PAX6
Entrez
Uniprot