Pax2 Antibody - Azide and BSA Free

Western Blot: Pax2 Antibody [NBP2-99013] - Lane A: K562 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:44 kDa

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution.

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in human kidney with rabbit polyclonal antibody at 1:1000 dilution.

Immunohistochemistry-Paraffin: Pax2 Antibody [NBP2-99013] - Staining of human Pax2 in human renal carcinoma with rabbit polyclonal antibody at 1:1000.

Immunoprecipitation: Pax2 Antibody [NBP2-99013] - Pax2 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 4 ul anti-Pax2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Pax2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 45 kDa

Immunocytochemistry/Immunofluorescence: Pax2 Antibody [NBP2-99013] - Immunofluorescence staining of Pax2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99013 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Pax2 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Pax2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAX2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 0.5-2uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Pax2 Antibody - Azide and BSA Free

• paired box 2
• paired box gene 2
• paired box homeotic gene 2
• paired box protein Pax-2
• Pax2

Background

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX2