Patched 1/PTCH Antibody

Images

 
Western Blot: Patched 1/PTCH Antibody [NBP1-71662] - Analysis of Patched 1 in human skeletal muscle protein.
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and ...read more
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody [NBP1-71662] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse lung using 1:50 dilution of PTCH antibody with HRP-DAB detection and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.22 mg/ml

Patched 1/PTCH Antibody Summary

Immunogen
A genomic peptide made to a C-terminal region of the human Patched 1 protein (within residues 1200-1350). [Swiss-Prot Q13635]
Localization
Membrane, Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTCH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500
  • Immunohistochemistry 1:50 - 1:250
  • Immunohistochemistry-Paraffin 1:50 - 1:250
Application Notes
This Patched 1 antibody is useful in Western blot, where a band is seen ~161 kDa.
Positive Control
Lung Lysate (NB820-59240)
Skeletal Muscle Lysate (NB820-59253)
Publications
Read Publications using
NBP1-71662 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.22 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Patched 1/PTCH Antibody

  • BCNS
  • BCNSFLJ42602
  • FLJ26746
  • HPE7
  • mes
  • NBCCS
  • patched (Drosophila) homolog
  • Patched 1
  • patched homolog (Drosophila)
  • patched homolog 1 (Drosophila)
  • patched homolog 1
  • protein patched homolog 1
  • PTC
  • ptc1
  • PTCH protein +12b
  • PTCH protein +4'
  • PTCH protein -10
  • PTCH
  • PTCH1
  • PTCH11

Background

Patched 1 (PTCH1) acts as a receptor for SHH, IHH (indian hedgehog) and DHH (desert hedgehog) in SHH/sonic hedgehog signaling pathway which is critical in embryonic development, tissue patterning, and cell-fate decisions. SHH pathway activation is triggered by binding of SHH to PTCH1 (glycosylation being necessary for binding), which in the absence of SHH suppresses the activity of SMO (Smoothened protein), and this SHH- PTCH1 binding or mutational inactivation of PTCH1 relieves the inhibition of SMO culminating in the activation of one or more of GLI1 transcription factors that regulate the expression of downstream targets. PTCH1 mutations or its aberrant expression may leads to developmental defects and a cancer-prone phenotype. Mice partially deficient in functional PTCH1 exhibit developmental abnormalities that are comparable to those of nevoid basal cell carcinoma syndrome patients and additionally, these mice develop tumors such as medulloblastomas, rhabdomyosarcomas, and basal cell carcinomas following UV or ionizing radiation exposure. PTCH1 germline mutations in humans are responsible for nevoid basal cell carcinoma syndrome, also called Gorlin syndrome characterized by various developmental malformations and a high predisposition to skin basal cell carcinoma. Besides basal cell nevus syndrome (BCNS), defects in PTCH1 have been linked to sporadic basal cell carcinoma (BCC) and holoprosencephaly type 7 (HPE7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Patched 1/PTCH Antibody (NBP1-71662)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for Patched 1/PTCH Antibody (NBP1-71662): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Patched 1/PTCH Antibody (NBP1-71662). (Showing 1 - 1 of 1 FAQs).

  1. I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?
    • NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71662AF405
Alexa Fluor 488 Labeled NBP1-71662AF488
Alexa Fluor 647 Labeled NBP1-71662AF647
Alexa Fluor 700 Labeled NBP1-71662AF700
Biotin Labeled NBP1-71662B
DyLight 405 Labeled NBP1-71662V
DyLight 488 Labeled NBP1-71662G
DyLight 550 Labeled NBP1-71662R
DyLight 650 Labeled NBP1-71662C
FITC Labeled NBP1-71662F
HRP Labeled NBP1-71662H

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Bioinformatics Tool for Patched 1/PTCH Antibody (NBP1-71662)

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Diseases for Patched 1/PTCH Antibody (NBP1-71662)

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Pathways for Patched 1/PTCH Antibody (NBP1-71662)

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PTMs for Patched 1/PTCH Antibody (NBP1-71662)

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Research Areas for Patched 1/PTCH Antibody (NBP1-71662)

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Blogs on Patched 1/PTCH

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Bioinformatics

Gene Symbol PTCH1
Entrez
Uniprot