![Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0003.jpg "Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Rat Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.")
| Reactivity | Hu, Mu, Rt, Ca, Eq, PmSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit NCOA4 Antibody - BSA Free (NBP3-18136) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Antibody was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4. |
| Predicted Species | Monkey (100%), Equine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NCOA4 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NCOA4 |
![Immunocytochemistry/Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with NBP3-18136 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-18136-img0004.jpg "Immunocytochemistry/Immunofluorescence: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling NCOA4 with NBP3-18136 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).")
![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18136-img0006.jpg "Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0001.jpg "Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.")
![Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Western-Blot-NBP3-18136-img0002.jpg "Western Blot: NCOA4 Antibody - BSA Free [NBP3-18136] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: NCOA4 NBP3-18136, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.")
![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/NCOA4-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP3-18136-img0005.jpg "Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded human mouse tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/NCOA4 Antibody - BSA Free-Immunohistochemistry-Paraffin-NBP3-18136-img0007.jpg "Immunohistochemistry-Paraffin: NCOA4 Antibody - BSA Free [NBP3-18136] - Immunohistochemical analysis of paraffin-embedded rat tissue using anti-NCOA4 antibody (NBP3-18136) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")