Western Blot: B-Raf Antibody [NBP1-89942] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human cell line A-431Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: B-Raf Antibody [NBP1-89942] - Staining of human cell line U-251MG shows positivity in nucleus & cytoplasm.
Immunohistochemistry-Paraffin: B-Raf Antibody [NBP1-89942] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
Western Blot: B-Raf Antibody [NBP1-89942] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).
Simple Western: B-Raf Antibody [NBP1-89942] - Simple Western lane view shows a specific band for BRAF in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions ...read more
Simple Western: B-Raf Antibody [NBP1-89942] - Electropherogram image(s) of corresponding Simple Western lane view. B-Raf antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).
Hu, Mu, RtSpecies Glossary
WB, Simple Western, ICC/IF, IHC, IHC-P
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
This antibody was developed against Recombinant Protein corresponding to amino acids:PIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSS
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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You can't be without me - SNF5 The protein encoded by SNF5 is a component of the chromatin-remodeling protein complex responsible for relieving repressive chromatin structures by allowing the transcriptional machinery to access targets more effectively. SNF5 has been found to be a... Read full blog post.