PASK Antibody - BSA Free

Western Blot: PASK Antibody [NBP2-19704] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PASK Antibody [NBP2-19704] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PASK Antibody [NBP2-19704] - xenograft, using PASK antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PASK Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human PASK. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PASK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 143 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PASK Antibody - BSA Free

• DKFZp434O051
• EC 2.7.11.1
• hPASK
• KIAA0135DKFZp686P2031
• PAS domain containing serine/threonine kinase
• PAS-kinase
• PASKINPAS domain-containing serine/threonine-protein kinase
• PAS-serine/threonine kinase
• STK37

Background

PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PASK is an evolutionarily conserved protein present in yeast, flies, and mammals.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PASK
• Uniprot:
  • Q96RG2(Human)