PAK6 Antibody - BSA Free

Western Blot: PAK6 Antibody [NBP1-76632] - Raji lysate with PAK6 antibody at (A) 1, (B) 2, and (C) 4 u/mL.

Immunocytochemistry/ Immunofluorescence: PAK6 Antibody - BSA Free [NBP1-76632] - Immunofluorescence of PAK6 in Raji cells with PAK6 antibody at 10 ug/mL.

Immunocytochemistry/ Immunofluorescence: PAK6 Antibody - BSA Free [NBP1-76632] - Immunocytochemistry of PAK6 in Raji cells with PAK6 antibody at 10 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PAK6 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK6. The immunogen is located within amino acids 260 - 310 of PAK6. Amino Acid Squence: PSSSKPGPPPQSK
• Specificity: PAK6 antibody is predicted to not cross-react with other PAK family proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK6
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 1-4 ug/ml
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PAK6 Peptide (NBP1-76632PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PAK6 Antibody - BSA Free

• EC 2.7.11
• p21 protein (Cdc42/Rac)-activated kinase 6
• p21(CDKN1A)-activated kinase 6
• p21-activated kinase 6
• p21-activated protein kinase 6
• PAK-5
• PAK5EC 2.7.11.1
• PAK6
• PAK-6
• serine/threonine-protein kinase PAK 6

Background

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAK6 Antibody (NBP1-76632)(1)

Publication using NBP1-76632

• Raja R, Sahasrabuddhe NA, Radhakrishnan A et al. Chronic exposure to cigarette smoke leads to activation of p21 (RAC1)-activated kinase 6 (PAK6) in non-small cell lung cancer cells. Oncotarget. 2016-09-20 [PMID: 27542207] (WB, Human)

Bioinformatics

• Gene Symbol: PAK6
• Uniprot:
  • NP_064553(Human)