pan Cadherin Antibody (CH-19)

Western Blot: pan Cadherin Antibody (CH-19) [NB100-1907] - Analysis of pan Cadherin Antibody (CH-19) in Madin Darby Bovine Kidney (MDBK) extract.

Immunohistochemistry-Paraffin: pan Cadherin Antibody (CH-19) [NB100-1907] - Analysis of pan Cadherin formalin-fixed paraffin embedded human breast carcinoma.

Product Discontinued

pan Cadherin Antibody (CH-19) Summary

• Immunogen: Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
• Localization: Cell membrane.
• Marker: Plasma Membrane Marker
• Specificity: Monoclonal Anti-Pan Cadherin antibody reacts extensively with all known members of the cadherin famil
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500
  • Microarray
  • Simple Western
  • Western Blot 1:100-1:2000
• Application Notes: The antibody reacts using frozen sections, protease-digested, formalin-fixed paraffin-embedded, Bouin's-fixed and acetone-fixed tissue sections.
  See Simple Western Antibody Database for Simple Western validation: Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:1000; matrix was 12-230 kDa.
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  E-Cadherin Overexpression Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for pan Cadherin Antibody (CH-19)

• Arc-1
• Cadherin (pan)
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDHE
• cell-CAM 120/80
• ECAD
• E-cadherin
• Epithelial cadherin
• LCAM
• UVOE-Cadherin
• uvomorulin

Background

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+-dependent cell-cell adhesion.1,2,3 The N-terminal part of these molecules is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for pan Cadherin Antibody (NB100-1907)(4)

Publications using NB100-1907

• Zeitz MJ, Calhoun PJ, James CC et al. Dynamic UTR Usage Regulates Alternative Translation to Modulate Gap Junction Formation during Stress and Aging Cell Rep 2019-05-28 [PMID: 31141695] (ICC/IF, Mouse)
• Sroubek J, Krishnan Y, McDonald TV. Sequence and structure-specific elements of HERG mRNA determine channel synthesis and trafficking efficiency. FASEB J. 2013-08-01 [PMID: 23608144] (WB, Human)
• Krishnan Y, Zheng R, Walsh C, Tang Y, McDonald TV. Partially dominant mutant channel defect corresponding with intermediate LQT2 phenotype. Pacing Clin Electrophysiol;35(1):3-16. 2012-01-01 [PMID: 21951015] (ICC/IF, Mouse)
• Orthmann-Murphy JL, Salsano E, Abrams CK et al. Hereditary spastic paraplegia is a novel phenotype for GJA12/GJC2 mutations. Brain;132(Pt 2):426-38. 2009-02-01 [PMID: 19056803]

Bioinformatics

• Gene Symbol: CDH1