pan Cadherin Antibody (CH-19)

Images

 
Western Blot: pan Cadherin Antibody (CH-19) [NB100-1907] - Analysis of pan Cadherin Antibody (CH-19) in Madin Darby Bovine Kidney (MDBK) extract.
Immunohistochemistry-Paraffin: pan Cadherin Antibody (CH-19) [NB100-1907] - Analysis of pan Cadherin formalin-fixed paraffin embedded human breast carcinoma.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Gt, Ha, Rb, Sh, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, MiAr
Clone
CH-19
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

pan Cadherin Antibody (CH-19) Summary

Immunogen
Synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH.
Localization
Cell membrane.
Marker
Plasma Membrane Marker
Specificity
Monoclonal Anti-Pan Cadherin antibody reacts extensively with all known members of the cadherin famil
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDH1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500
  • Microarray
Application Notes
The antibody reacts using frozen sections, protease-digested, formalin-fixed paraffin-embedded, Bouin's-fixed and acetone-fixed tissue sections.
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
E-Cadherin Lysate (NBL1-09024)
Publications
Read Publications using
NB100-1907 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
15mM Sodium Azide
Purity
Unpurified

Alternate Names for pan Cadherin Antibody (CH-19)

  • Arc-1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDHE
  • cell-CAM 120/80
  • ECAD
  • E-cadherin
  • Epithelial cadherin
  • LCAM
  • UVOE-Cadherin
  • uvomorulin

Background

Cadherins are members of a multigene family of single chain glycoprotein receptors mediating Ca2+-dependent cell-cell adhesion.1,2,3 The N-terminal part of these molecules is exposed on the external cell surface and contains the putative homophilic binding sites. This is followed by a typical single transmembrane sequence and usually a cytoplasmic C-terminal tail which mediates interaction with the microfilament system through molecules such as catenins, plakoglobin, vinculin, and

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for pan Cadherin Antibody (NB100-1907)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for pan Cadherin Antibody (NB100-1907) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDH1