PADI2 Antibody

PADI2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain (cortex) using Sheep Anti-Human PADI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7257) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PADI2 Antibody Summary

• Immunogen: E. coli-derived recombinant human PADI2
  Met1-Ala115
  Accession # Q9Y2J8
• Specificity: Detects human PADI2 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PADI2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PADI2 Antibody

• EC 3.5.3.15
• KIAA0994PDI2PAD2
• PAD2
• PAD-H19
• PADI2
• PDI2
• peptidlyarginine deiminase type II
• peptidyl arginine deiminase, type II
• Peptidylarginine Deiminase II
• protein arginine deiminase
• Protein-arginine deiminase type II
• protein-arginine deiminase type-2

Background

PADI2 (Protein-arginine deiminase type 2; also PAD2, PDI2 and PAD-H19) is a 72-76 kDa member of the PAD family of proteins. It is widely expressed, being found in macrophages, astrocytes, select neurons, skeletal muscle and bronchial epithelial cells, and keratinocytes. PADI2 catalyzes the Ca++-dependent conversion of guanidino groups (NH2-C[=NH]-NH−) to ureido groups (NH2-C[=O]-NH-) on peptidylArg, a posttranslational process known as citrullination. Human PADI2 is 665 amino acids (aa) in length. It contains an N-terminal PAD domain (aa 1-114), a middle PAD-M domain (aa 116-274), and a catalytic region (aa 279-665). Over aa 1-115, human PADI2 shares 90% aa sequence identity with mouse PADI2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PADI2
• Uniprot:
  • Q9Y2J8(Human)