PRMT1 Antibody

Western blot shows lysates of 293T human embryonic kidney cell line and Hepa 1-6 mouse hepatoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human PRMT1 Polyclonal Antibody (Catalog # AF6016) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PRMT1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PRMT1 Antibody Summary

• Immunogen: E. coli-derived recombinant human PRMT1
  Lys218-Arg361
  Accession # Q99873
• Specificity: Detects human and mouse PRMT1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRMT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRMT1 Antibody

• ANM1
• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.125
• EC 2.1.1.77
• HCP1
• Histone-arginine N-methyltransferase PRMT1
• HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2
• HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae)
• HMT1
• HMT2
• HRMT1L2
• HRMT1L2HMT1 hnRNP methyltransferase-like 2
• Interferon receptor 1-bound protein 4
• IR1B4
• IR1B4protein arginine N-methyltransferase 1
• PRMT1
• protein arginine methyltransferase 1

Background

PRMT/PRMT1 (Protein-Arginine Methyltransferase 1; also HRMT1L2 and HMT2) is a 42‑45 kDa type I member of the arginine N‑methyltransferase family of enzymes. It is ubiquitously expressed and dimethylates single arginine residue nitrogen atoms in a distributive manner (i.e.-with substrate release following each methlyation step). PRMT1 forms homodimers, and heterodimers with PRMT3, and exists as part of a 300‑400 kDa complex in vivo. Human PRMT1 is 361 amino acids in length and contains one adenosyl-L-methionine binding site (aa 85‑153) and a phosphorylation site at Tyr299. There are multiple splice variants that show distinct substrate specificities. There is an alternate start site 10 aa upstream of the standard start site, and a single Met, plus a five aa and 11 aa substiuition for aa 1‑19. One isoform has a 2 aa substitution for aa 1‑20 accompanied by a deketion of aa 52‑67. Over aa 218‑361, human PRMT1 is 100% identical in aa sequence to mouse PRMT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRMT1 Antibody (AF6016)(2)

Publications using AF6016

• Corina E. Antal, Tae Gyu Oh, Stefan Aigner, En-Ching Luo, Brian A. Yee, Tania Campos et al. A super-enhancer-regulated RNA-binding protein cascade drives pancreatic cancer Nat Commun 9/6/2023 [PMID: 37673892]
• M Krzystek-K, B Szcz??niak, I Szczuka, P Fortuna, M Zawadzki, A Kubiak, M Mierzcha?a, MG Fleszar, ? Lewandowsk, P Serek, N Jamrozik, K Neubauer, J Wi?niewski, R Kempi?ski, W Witkiewicz, I Bednarz-Mi L-Arginine/Nitric Oxide Pathway Is Altered in Colorectal Cancer and Can Be Modulated by Novel Derivatives from Oxicam Class of Non-Steroidal Anti-Inflammatory Drugs Cancers (Basel), 2020-09-11;12(9):. 2020-09-11 [PMID: 32932854] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PRMT1
• Uniprot:
  • Q99873()