p66 beta Antibody

Western Blot: p66 beta Antibody [NB100-60646] - Detection of Human p66beta/GATAD2B on HeLa whole cell lysate using NB100-60646. p66beta/GATAD2B was also immunoprecipitated by rabbit anti-p66beta/GATAD2B antibodies NB100-60645 and NB100-60647.

Immunohistochemistry: p66 beta Antibody [NB100-60646] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- p66beta/GATAD2B used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: p66 beta Antibody [NB100-60646] - Human small cell lung cancer. Antibody: Affinity purified rabbit antip66beta/ GATAD2B used at a dilution of 1:250.

Immunohistochemistry-Paraffin: p66 beta Antibody [NB100-60646] - Human small cell lung cancer. Antibody: Affinity purified rabbit antip66beta/GATAD2B used at a dilution of 1:250.

Immunohistochemistry-Paraffin: p66 beta Antibody [NB100-60646] - Human small cell lung cancer. Antibody: Affinity purified rabbit antip66beta/GATAD2B used at a dilution of 1:250.

Immunohistochemistry-Paraffin: p66 beta Antibody [NB100-60646] - Human small cell lung cancer. Antibody: Affinity purified rabbit antip66beta/GATAD2B used at a dilution of 1:250.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

p66 beta Antibody Summary

• Description: Novus Biologicals Rabbit p66 beta Antibody (NB100-60646) is a polyclonal antibody validated for use in IHC, WB, IP and ChIP. Anti-p66 beta Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human transcription repressor p66 beta component of the MeCP1 complex (GATA zinc finger domain containing 2B ) using the numbering given in entry NP_065750.1 (GeneID
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GATAD2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Use in ChIP reported in scientific literature (PMID:34260936). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  p66 beta Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p66 beta Antibody

• FLJ37346
• GATA zinc finger domain containing 2B
• GATA zinc finger domain-containing protein 2B
• KIAA1150
• MGC138257
• MGC138285
• p66/p68
• P66beta
• RP11-216N14.6
• transcription repressor p66 beta component of the MeCP1 complex
• transcriptional repressor p66-beta

Background

P66beta/GATAD2B interacts with MBD2 (methyl-CpG-binding domain 2) and MBD3 (methyl-CpG-binding domain 3) and participates in transcriptional repression in association with the MBD2/NuRD and MBD3/NuRD histone deacetylase complexes. P66beta/GATAD2B is SUMOylated in vivo and its repressive activity has been shown to be enhanced by SUMOylation. Alternate names for P66beta/GATAD2B include transcriptional repressor p66 beta, p66/p68, GATA zinc-finger domain-containing protein 2B, FLJ37346, and KIAA1150.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p66 beta Antibody (NB100-60646)(2)

Publications using NB100-60646

• Kim B, Luo Y, Zhan X Et Al. Neuronal activity-induced BRG1 phosphorylation regulates enhancer activation Cell reports 2021-07-13 [PMID: 34260936] (Chemotaxis, WB)
• Okkelman IA, Sukaeva AZ, Kirukhina EV et al. Nuclear translocation of lysyl oxidase is promoted by interaction with transcription repressor p66B Cell Tissue Res. 2014-08-15 [PMID: 25118846]

Bioinformatics

• Gene Symbol: GATAD2B
• Entrez:
  • 57459(Human)
• Uniprot:
  • NP_065750.1(Human)