HMBOX1 Antibody - BSA Free

Western Blot: HMBOX1 Antibody [NBP1-31316] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: HMBOX1 Antibody [NBP1-31316] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMBOX1 protein stained by HMBOX1 antibody [N3C3] (NBP1-31316) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (NBP1-33273) diluted at 1:10000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: HMBOX1 Antibody [NBP1-31316] - SW480 xenograft , using Homeobox-containing protein 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, Bv, Ch, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HMBOX1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HMBOX1 Antibody - BSA Free (NBP1-31316) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-HMBOX1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HMBOX1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (98%), Bovine (100%), Chicken (97%), Xenopus (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMBOX1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: IP-Assay dependent
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HMBOX1 Antibody - BSA Free

• FLJ21616
• HNF1LA
• homeobox containing 1
• homeobox-containing protein 1
• homeobox-containing protein PBHNF
• PBHNF

Background

HMBOX1, also known as Homeobox-containing protein 1, is a 47kDa 429 amino acid protein with four isoforms produced by alternative splicing. The gene can be found in the nucleus and the cytoplasm where it acts as a transcriptional repressor. Current research on HMBOX1 is being conducted in relation to bronchopulmonary dysplasia, immunodeficiency and prostatitis. HMBOX1 is linked to the MRNA splicing, nuclear transport, stem cell differentiation, tube formation and transport pathways where it interacts with SUMO2 and ENTPD2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HMBOX1 Antibody (NBP1-31316)(1)

Publication using NBP1-31316

• Lee JH, Hong J, Zhang Z et al. Regulation of telomere homeostasis and genomic stability in cancer by N (6)-adenosine methylation (m(6)A) Science Advances 2021-07-30 [PMID: 34321211] (Western Blot)

Bioinformatics

• Gene Symbol: HMBOX1