Home » FoxP2 » FoxP2 Antibodies » FoxP2 Antibody [Unconjugated]

FoxP2 Antibody [Unconjugated]

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Western blot shows lysates of A172 human glioblastoma cell line and human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of A172 human glioblastoma cell line and human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
FoxP2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) for 3 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5647-SP
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FoxP2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FoxP2 isoform 1
Ala640-Glu715
Accession # O15409
Specificity
Detects human and mouse FoxP2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
FOXP2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5647 in the following applications:

  • ICC
    1 publication
  • IHC
    6 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP2 Antibody [Unconjugated]

  • CAG repeat protein 44
  • CAGH44
  • CAGH44TNRC10forkhead box protein P2
  • DKFZp686H1726
  • forkhead box P2
  • forkhead/winged-helix transcription factor
  • FoxP2
  • SPCH1
  • TNRC10
  • trinucleotide repeat containing 10
  • Trinucleotide repeat-containing gene 10 protein

Background

FoxP2 (Forkhead box protein P2; also CAGH44) is an 85‑95 kDa member of the winged helix transcription factor gene family. It is a transcriptional repressor that is expressed principally in gut and lung epithelium, and in neurons involved in speech and language. Human FoxP2 is 715 amino acids (aa) in length. It contains a huge poly-Gln region (aa 123‑231), a C2H2 Zn-finger domain (aa 346‑371), a Leu-zipper segment (aa 388‑409) and a forkhead DNA binding domain (aa 504‑594). FoxP2 both homodimerizes and heterodimerizes with FoxP1 and P4, and binds CTBP1. There are multiple splice variants. There is an alternate start site at Met93, an 11 aa substitution for aa 57‑715, a 25 aa insert after Gln86, an 11 aa substitution for aa 133‑715, and a ten aa substitution for aa 423‑715. Over aa 587‑715, human and mouse FoxP2 are (100%) identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP2 Antibody (AF5647)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ICC, IHC.


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ICC
(1)
IHC
(6)
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Mouse
(4)
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(3)
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Showing Publications 1 - 8 of 8.
Publications using AF5647 Applications Species
RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans Cell Rep, 2020;33(8):108425. 2020 [PMID: 33238113] (IHC, Rat) IHC Rat
AH Pool, T Wang, DA Stafford, RK Chance, S Lee, J Ngai, Y Oka The cellular basis of distinct thirst modalities Nature, 2020;0(0):. 2020 [PMID: 33057193] (IHC, Mouse) IHC Mouse
S Lee, V Augustine, Y Zhao, H Ebisu, B Ho, D Kong, Y Oka Chemosensory modulation of neural circuits for sodium appetite Nature, 2019;568(7750):93-97. 2019 [PMID: 30918407] (IHC, Mouse) IHC Mouse
CF Yang, JL Feldman Efferent projections of excitatory and inhibitory preB�tzinger complex neurons J. Comp. Neurol., 2018;0(0):. 2018 [PMID: 29473167] (IHC, Mouse) IHC Mouse
D Stani?, RR Dhingra, M Dutschmann Expression of the transcription factor FOXP2 in brainstem respiratory circuits of adult rat is restricted to upper-airway pre-motor areas Respir Physiol Neurobiol, 2018;250(0):14-18. 2018 [PMID: 29414420] (IHC, Rat) IHC Rat
AM Verstegen, V Vanderhors, PA Gray, ML Zeidel, JC Geerling Barrington's nucleus: Neuroanatomic landscape of the mouse pontine micturition center J. Comp. Neurol, 2017;0(0):. 2017 [PMID: 28340519] (IHC, Mouse) IHC Mouse
Joel Geerling K�lliker-Fuse GABAergic and glutamatergic neurons project to distinct targets J. Comp. Neurol, 2016;0(0):. 2016 [PMID: 28032634] (ICC, Rat) ICC Rat
HSD2 neurons in the hindbrain drive sodium appetite Nat. Neurosci, 2016;0(0):. 2016 [PMID: 27918529]

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Bioinformatics

Gene Symbol FOXP2
Uniprot