p63/TP73L Antibody [Biotin]

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="p63/TP73L was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

p63/TP73L was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # <a class=

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin

p63/TP73L Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human p63/TP73L
  Met40-Cys339
  Accession # Q9H3D4
• Specificity: Detects p63/TP73L in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TP63
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p63/TP73L Antibody [Biotin]

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• P73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Background

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p63/TP73L Antibody (BAF1916)(5)

Publications using BAF1916

• Frum, T;Hsu, PP;Hein, RFC;Conchola, AS;Zhang, CJ;Utter, OR;Anand, A;Zhang, Y;Clark, SG;Glass, I;Sexton, JZ;Spence, JR; Opposing roles for TGF?- and BMP-signaling during nascent alveolar differentiation in the developing human lung bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205521] (IHC, Human)
• Renee F.C. Hein, Joshua H. Wu, Emily M. Holloway, Tristan Frum, Ansley S. Conchola, Yu-Hwai Tsai et al. R-SPONDIN2+ mesenchymal cells form the bud tip progenitor niche during human lung development Developmental Cell 7/1/2022 [PMID: 35679862]
• Yasufumi Koterazawa, Michiyo Koyanagi-Aoi, Keiichiro Uehara, Yoshihiro Kakeji, Takashi Aoi Retinoic acid receptor gamma activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium Journal of Gastroenterology 8/1/2020 [PMID: 32556644]
• Alyssa J. Miller, Qianhui Yu, Michael Czerwinski, Yu-Hwai Tsai, Renee F. Conway, Angeline Wu et al. In Vitro and In Vivo Development of the Human Airway at Single-Cell Resolution Developmental Cell 4/1/2020 [PMID: 32109386]
• AJ Miller, BR Dye, D Ferrer-Tor, DR Hill, AW Overeem, LD Shea, JR Spence Generation of lung organoids from human pluripotent stem cells in vitro Nat Protoc, 2019-02-01;14(2):518-540. 2/1/2019 [PMID: 30664680]

Bioinformatics

• Gene Symbol: TP63
• Uniprot:
  • Q9H3D4()