CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1916-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08660 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08736 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43892 | |||
| BSA Free | NBP3-05501-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08776 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93323-0.02ml | |||
NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for ![Analysis of p73 and p63 co-expression in human and murine skin.(A) Scatter plot of TP63 versus TP73 RNA-seq expression [units = transcripts per million (TPM)] by human tissue type (n = 37) from the Human Protein Atlas (172 total samples) [30]. Mean expression (TPM + 0.1) for each tissue is plotted on a log2 scale with a LOESS smooth local regression line (gray). Correlation between TP63 and TP73 was quantified using Spearman’s rank correlation coefficient (rs). (B) Representative micrographs of H&E and immunofluorescence (IF) staining on serial human (top) and mouse (bottom) skin sections; DAPI (blue), p63 alpha (green), and p73 (red). Regions of the skin in micrographs are labeled as: interfollicular epidermis (IFE), hair follicle (HF), outer root sheath (ORS), HF bulge (Bu), hair bulb (HB), sebaceous gland (SG), hair shaft (HS), and arrector pili muscle (APM). Scale bars represent 200 μm for human and 50 μm for murine tissue. See also S1 and S2 Figs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31216312), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af1916_human-p63-tp73l-affinity-purified-polyclonal-ab-41202412401237.jpg)
human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue). " class="big_thumb" />
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Immunohistochemistry-Paraffin-NBP3-07430-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).")
![Flow Cytometry: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Flow-Cytometry-NBP3-07430-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human skin stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Immunohistochemistry-Paraffin-NBP3-07430-img0002.jpg)
![Western Blot: TAp63 Antibody (TAp63-4.1) [NBP3-11703] - Analysis, confirming specificity of mouse monoclonal antibody TAp63-4.1 for TAp63 isoform of p63 protein. Cell lysates from particular transfectants of TAp63alpha, TAp63gamma, deltaNp63alpha, TAp73alpha, and deltaNp73alpha were analysed.](http://images.novusbio.com/fullsize/TAp63-Antibody-TAp63-4-1-Western-Blot-NBP3-11703-img0001.jpg "Western Blot: TAp63 Antibody (TAp63-4.1) [NBP3-11703] - Analysis, confirming specificity of mouse monoclonal antibody TAp63-4.1 for TAp63 isoform of p63 protein. Cell lysates from particular transfectants of TAp63alpha, TAp63gamma, deltaNp63alpha, TAp73alpha, and deltaNp73alpha were analysed.")
![Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-27820241631652.jpg "Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p63/TP73L antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32703, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-298202413585578.jpg)
![Immunocytochemistry/ Immunofluorescence: p63/TP73L Antibody (SC06-31) [NBP3-32703] - ICC staining of p63/TP73L in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32703, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-2920241192350.jpg)
![Flow Cytometry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Flow cytometric analysis of p63/TP73L was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32703, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-29202416453240.jpg)
![Western Blot: p40/deltaNp63 Antibody (PDH0-06) [NBP3-32695] - Western blot analysis of p40/deltaNp63 on A431 cell lysates with Rabbit anti-p40/deltaNp63 antibody (NBP3-32695) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 77 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32695) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32695_rabbit-p40-deltanp63-mab-pdh0-06-278202415295573.jpg "Western Blot: p40/deltaNp63 Antibody (PDH0-06) [NBP3-32695] - Western blot analysis of p40/deltaNp63 on A431 cell lysates with Rabbit anti-p40/deltaNp63 antibody (NBP3-32695) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 77 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32695) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p40/deltaNp63 Antibody (PDH0-06) [NBP3-32695] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-p40/deltaNp63 antibody (NBP3-32695) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32695) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32695_rabbit-p40-deltanp63-mab-pdh0-06-29820241492665.jpg)
![Flow Cytometry: p40/deltaNp63 Antibody (PDH0-06) [NBP3-32695] - Flow cytometric analysis of A431 cells labeling p40/deltaNp63.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32695, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32695_rabbit-p40-deltanp63-mab-pdh0-06-29202416453271.jpg)
![Western Blot: TP63 Antibody (87C553) [NB100-56549] - analysis of p63 in mouse 1) placenta and 2) thymus using antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/TP63-Antibody-87C553-Western-Blot-NB100-56549-img0003.jpg "Western Blot: TP63 Antibody (87C553) [NB100-56549] - analysis of p63 in mouse 1) placenta and 2) thymus using antibody at 3 ug/ml.")
![Western Blot: TP63 Antibody (87C553) [NB100-56549] - analysis of p63 in A549 lysate using p63 antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/TP63-Antibody-87C553-Western-Blot-NB100-56549-img0002.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p63/TP73L Mouse Monoclonal Antibody (TP63/2428).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-TP63-2428-Immunohistochemistry-Paraffin-NBP3-07443-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with p63/TP73L Mouse Monoclonal Antibody (TP63/2428).")
![Flow Cytometry: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Mouse Monoclonal Antibody (TP63/2428) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-TP63-2428-Flow-Cytometry-NBP3-07443-img0002.jpg)
![Protein Array: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Analysis of Protein Array containing more than 19,000 full-length human proteins using p63/TP73L Antibody (TP63/2428).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-TP63-2428-Protein-Array-NBP3-07443-img0005.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/2428) [NBP3-07443] - Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with p63/TP73L Mouse Monoclonal Antibody (TP63/2428).](http://images.novusbio.com/fullsize/nbp3-07443_mouse-monoclonal-p63-tp73l-antibody-tp63-2428-17720241003239.jpg)
![Western Blot: p63/TP73L Antibody (CL3748) [NBP3-05501] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-CL3748-Western-Blot-NBP3-05501-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: p63/TP73L Antibody (CL3748) [NBP3-05501] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human prostate shows nuclear immunoreactivity in basal epithelial cells.](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-CL3748-Immunohistochemistry-Paraffin-NBP3-05501-img0006.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-CL3748-Immunohistochemistry-Paraffin-NBP3-05501-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human liver shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-CL3748-Immunohistochemistry-Paraffin-NBP3-05501-img0002.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human lung cancer (adenocarcinoma) shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-CL3748-Immunohistochemistry-Paraffin-NBP3-05501-img0003.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human placenta shows nuclear positivity in a subset of cells.](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-CL3748-Immunohistochemistry-Paraffin-NBP3-05501-img0004.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (CL3748) [NBP3-05501] - Staining of human prostate cancer shows absence of nuclear immunoreactivity in tumor cells, while it is present in the adjacent normal a basal epithelial cells.](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-CL3748-Immunohistochemistry-Paraffin-NBP3-05501-img0005.jpg)
![Western Blot: p40/deltaNp63 Antibody (PDH0-02) [NBP3-32694] - Western blot analysis of p40/deltaNp63 on A431 cell lysates with Rabbit anti-p40/deltaNp63 antibody (NBP3-32694) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 77 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32694) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32694_rabbit-p40-deltanp63-mab-pdh0-02-2882024142263.jpg "Western Blot: p40/deltaNp63 Antibody (PDH0-02) [NBP3-32694] - Western blot analysis of p40/deltaNp63 on A431 cell lysates with Rabbit anti-p40/deltaNp63 antibody (NBP3-32694) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 77 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 3 minutes;<br/><br/> 8% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32694) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p40/deltaNp63 Antibody (PDH0-02) [NBP3-32694] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-p40/deltaNp63 antibody (NBP3-32694) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32694) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32694_rabbit-p40-deltanp63-mab-pdh0-02-29820241435269.jpg)
![Immunocytochemistry/ Immunofluorescence: p40/deltaNp63 Antibody (PDH0-02) [NBP3-32694] - Immunocytochemistry analysis of A431 cells labeling p40/deltaNp63 with Rabbit anti-p40/deltaNp63 antibody (NBP3-32694) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-p40/deltaNp63 antibody (NBP3-32694) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32694_rabbit-p40-deltanp63-mab-pdh0-02-29202410484810.jpg)
![Immunocytochemistry/Immunofluorescence: p63/TP73L Antibody (ZR8) [NBP3-07454] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling p63/TP73L Recombinant Rabbit Monoclonal Antibody (ZR8) followed by goat anti-rabbit IgG-CF488 (green); phalloidin counterstain (red).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-ZR8-Immunocytochemistry-Immunofluorescence-NBP3-07454-img0002.jpg "Immunocytochemistry/Immunofluorescence: p63/TP73L Antibody (ZR8) [NBP3-07454] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling p63/TP73L Recombinant Rabbit Monoclonal Antibody (ZR8) followed by goat anti-rabbit IgG-CF488 (green); phalloidin counterstain (red).")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (ZR8) [NBP3-07454] - Formalin-fixed, paraffin-embedded human skin stained with p63/TP73L Recombinant Rabbit Monoclonal Antibody (ZR8).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-ZR8-Immunohistochemistry-Paraffin-NBP3-07454-img0004.jpg)
![Flow Cytometry: p63/TP73L Antibody (ZR8) [NBP3-07454] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Rabbit Monoclonal Antibody (ZR8) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-ZR8-Flow-Cytometry-NBP3-07454-img0001.jpg)
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (ZR8) [NBP3-07454] - Formalin-fixed, paraffin-embedded human skin stained with p63/TP73L Recombinant Rabbit Monoclonal Antibody (ZR8).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-ZR8-Immunohistochemistry-Paraffin-NBP3-07454-img0003.jpg)
![Western Blot: p63/TP73L Antibody - Azide and BSA Free [NBP2-93323] - Western blot analysis of various lysates, using p63/TP73L Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit.<br>Negative control (NC):MCF7<br>Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-93323_rabbit-polyclonal-p63-tp73l-antibody-4220251429046.jpg "Western Blot: p63/TP73L Antibody - Azide and BSA Free [NBP2-93323] - Western blot analysis of various lysates, using p63/TP73L Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit.<br>Negative control (NC):MCF7<br>Exposure time: 10s.")
![Immunohistochemistry: p63/TP73L Antibody - Azide and BSA Free [NBP2-93323] - Immunohistochemistry analysis of paraffin-embedded Mouse skin using p63/TP73L Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-93323_rabbit-polyclonal-p63-tp73l-antibody-422025143187.jpg)
![Immunohistochemistry: p63/TP73L Antibody - Azide and BSA Free [NBP2-93323] - Immunohistochemistry analysis of paraffin-embedded Rat skin using p63/TP73L Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-93323_rabbit-polyclonal-p63-tp73l-antibody-42202514264471.jpg)
![Immunocytochemistry/ Immunofluorescence: p63/TP73L Antibody - Azide and BSA Free [NBP2-93323] - Immunofluorescence analysis of human esophageal cancer using p63/TP73L Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93323_rabbit-polyclonal-p63-tp73l-antibody-42202514275291.jpg)
