Cytokeratin 14 Antibody (LL002)

Images

 
Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human skin stained with Biotin-conjugated Cytokeratin 14 Mouse Monoclonal Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype ...read more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002)
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Simple Western lane view shows a specific band for Cytokeratin 14 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 14 antibody was used at 10 ug/ml dilution of A431 lysates(s) respectively.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
LL002
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 14 Antibody (LL002) Summary

Immunogen
A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14. (Uniprot: P02533)
Localization
Cytoplasmic
Marker
Squamous Cell Marker
Specificity
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-34270 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34675)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 14 Antibody (LL002)

  • CK14
  • CK-14
  • Cytokeratin 14
  • cytokeratin-14
  • EBS3
  • EBS4
  • K14
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14
  • keratin, type I cytoskeletal 14
  • keratin-14
  • KRT14
  • NFJ

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 14 Antibody (NBP2-34270)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Flow, IHC-Fr.


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Flow
(1)
IHC-Fr
(1)
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Mouse
(1)
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Cytokeratin 14 Antibody (NBP2-34270). (Showing 1 - 1 of 1 FAQs).

  1. I received this antibody today and the data sheet recommends a concentration of 1-2ug/ml for IF however nowhere is the stock concentration listed so I don't know what dilution to do. Data sheet says check label and the label does not have the conc. The LOT number I received is 3861-1P150129 Please let me know what concentration the stock is.
    • I can confirm you that the the concentration of Lot# 3861-1P150129 of Cytokeratin 14 Antibody (LL002) [NBP2-34270] is 200ug/ml. This format of the antibody also contains 0.05% BSA and 0.05% Azide as stabalizer and preservative respectively. So you can try this antibody at 1:100 - 1:200 dilution range, and based on your pilot results on your particular samples, you can go for lower or higher dilution for obtaining the best signal.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT14
Uniprot