Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 ...read more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype ...read more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human prostate (10X) stained with Cytokeratin 14 MAb (LL002).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human prostate (20X) stained with Cytokeratin 14 MAb (LL002).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002)
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometry: Cytokeratin 14 Antibody (LL002) [Alexa Fluor® 488] [NBP2-34675AF488] - An intracellular stain was performed on HeLa cells with ...read more
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Simple Western lane view shows a specific band for Cytokeratin 14 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 14 antibody was used at 10 ug/ml dilution of A431 lysates(s) respectively.
Hu, Mu, RtSpecies Glossary
WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14
Squamous Cell Marker
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Protein A purified
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IHC-P: staining of formalin-fixed tissues requires retrieval of antigen by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes. ELISA: for coating, order NBP2-34675 (BSA free version of NBP2-34270). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-34270 in the following applications:
FAQs for Cytokeratin 14 Antibody (NBP2-34270). (Showing 1 - 1 of 1 FAQs).
I received this antibody today and the data sheet recommends a concentration of 1-2ug/ml for IF however nowhere is the stock concentration listed so I don't know what dilution to do. Data sheet says check label and the label does not have the conc. The LOT number I received is 3861-1P150129 Please let me know what concentration the stock is.
I can confirm you that the the concentration of Lot# 3861-1P150129 of Cytokeratin 14 Antibody (LL002) [NBP2-34270] is 200ug/ml. This format of the antibody also contains 0.05% BSA and 0.05% Azide as stabalizer and preservative respectively. So you can try this antibody at 1:100 - 1:200 dilution range, and based on your pilot results on your particular samples, you can go for lower or higher dilution for obtaining the best signal.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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