Cytokeratin 14 Antibody (LL002)


Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002)
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Simple Western lane view shows a specific band for Cytokeratin 14 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions more
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 14 antibody was used at 10 ug/ml dilution of A431 lysates(s) respectively.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
0.2 mg/ml

Order Details

Cytokeratin 14 Antibody (LL002) Summary

A synthetic peptide of 15 amino acid from the C-terminus of human Cytokeratin 14. (Uniprot: P02533)
Squamous Cell Marker
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
IgG3 Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Simple Western 10 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NBP2-34270 in the following applications:

Packaging, Storage & Formulations

Store at 4C.
10 mM PBS with 0.05% BSA
0.05% Sodium Azide
0.2 mg/ml
Protein A or G purified


200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34675)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Cytokeratin 14 Antibody (LL002)

  • CK14
  • CK-14
  • Cytokeratin 14
  • cytokeratin-14
  • EBS3
  • EBS4
  • K14
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14
  • keratin, type I cytoskeletal 14
  • keratin-14
  • KRT14
  • NFJ


Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu
Applications: ICC, IHC, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Ca, Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Bv, Ca, Ch, Hu, Mu, Po, Pm, Rt, Xp
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-Fr, IHC-P, In vivo, KO, Simple Western, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, KO, WB
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, Simple Western, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Species: Bv, Ca, Ch, Hu, Pm, Mu, Rb, Rt, Re, Ze
Applications: CyTOF-reported, Dual ISH-IHC, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single-Cell Western, WB
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: EnzAct
Species: Hu, Rt
Applications: IHC, IHC-P, WB
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: BA

Publications for Cytokeratin 14 Antibody (NBP2-34270)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: FLOW, IHC-Fr.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for Cytokeratin 14 Antibody (NBP2-34270) (0)

There are no reviews for Cytokeratin 14 Antibody (NBP2-34270). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Cytokeratin 14 Antibody (NBP2-34270). (Showing 1 - 1 of 1 FAQs).

  1. I received this antibody today and the data sheet recommends a concentration of 1-2ug/ml for IF however nowhere is the stock concentration listed so I don't know what dilution to do. Data sheet says check label and the label does not have the conc. The LOT number I received is 3861-1P150129 Please let me know what concentration the stock is.
    • I can confirm you that the the concentration of Lot# 3861-1P150129 of Cytokeratin 14 Antibody (LL002) [NBP2-34270] is 200ug/ml. This format of the antibody also contains 0.05% BSA and 0.05% Azide as stabalizer and preservative respectively. So you can try this antibody at 1:100 - 1:200 dilution range, and based on your pilot results on your particular samples, you can go for lower or higher dilution for obtaining the best signal.

Secondary Antibodies


Isotype Controls

Additional Cytokeratin 14 Products

Bioinformatics Tool for Cytokeratin 14 Antibody (NBP2-34270)

Discover related pathways, diseases and genes to Cytokeratin 14 Antibody (NBP2-34270). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytokeratin 14 Antibody (NBP2-34270)

Discover more about diseases related to Cytokeratin 14 Antibody (NBP2-34270).

Pathways for Cytokeratin 14 Antibody (NBP2-34270)

View related products by pathway.

PTMs for Cytokeratin 14 Antibody (NBP2-34270)

Learn more about PTMs related to Cytokeratin 14 Antibody (NBP2-34270).

Research Areas for Cytokeratin 14 Antibody (NBP2-34270)

Find related products by research area.

Blogs on Cytokeratin 14

There are no specific blogs for Cytokeratin 14, but you can read our latest blog posts.
Download our IHC Handbook

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Cytokeratin 14 Antibody (LL002) and receive a gift card or discount.


Gene Symbol KRT14