Cytokeratin 14 Antibody (LL002)

Images

 
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002).
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometry: Cytokeratin 14 Antibody (LL002) [Alexa Fluor® 488] [NBP2-34675AF488] - An intracellular stain was performed on HeLa cells with ...read more
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human prostate (10X) stained with Cytokeratin 14 MAb (LL002).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human prostate (20X) stained with Cytokeratin 14 MAb (LL002).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002)
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Simple Western lane view shows a specific band for Cytokeratin 14 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 14 antibody was used at 10 ug/ml dilution of A431 lysates(s) respectively.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
LL002
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 14 Antibody (LL002) Summary

Immunogen
A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14
Localization
Cytoplasmic
Marker
Squamous Cell Marker
Specificity
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
Isotype
IgG3
Clonality
Monoclonal
Host
Mouse
Gene
KRT14
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • Simple Western 10 ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2ug/ml
  • Immunoprecipitation 1-2ug/500ug protein
Application Notes
ELISA (For coating, order NBP2-34675 (BSA free version of NBP2-34270)). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-34270 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin 14 Antibody (LL002)

  • CK14
  • CK-14
  • Cytokeratin 14
  • cytokeratin-14
  • EBS3
  • EBS4
  • K14
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14
  • keratin, type I cytoskeletal 14
  • keratin-14
  • KRT14
  • NFJ

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22194
Species: Hu, Mu, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-61736
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, IHC, IHC-P
NB100-687
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
AF1916
Species: Hu
Applications: WB, IHC, ICC
NBP1-85571
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-16094
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KO
NB100-355
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Xp
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IF
NBP2-24482
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB
NBP1-88080
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
AF1095
Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow
NBP2-29429
Species: Hu, Mu, Rt, Bv, Ca, Ch, Pm, Rb, Re, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC, Flow-IC, Single Cell Western
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-85630
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-85632
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-67559
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-29461
Species: Hu, Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-38166
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Cytokeratin 14 Antibody (NBP2-34270)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Flow, IHC-Fr.


Filter By Application
Flow
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for Cytokeratin 14 Antibody (NBP2-34270) (0)

There are no reviews for Cytokeratin 14 Antibody (NBP2-34270). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 14 Antibody (NBP2-34270). (Showing 1 - 1 of 1 FAQs).

  1. I received this antibody today and the data sheet recommends a concentration of 1-2ug/ml for IF however nowhere is the stock concentration listed so I don't know what dilution to do. Data sheet says check label and the label does not have the conc. The LOT number I received is 3861-1P150129 Please let me know what concentration the stock is.
    • I can confirm you that the the concentration of Lot# 3861-1P150129 of Cytokeratin 14 Antibody (LL002) [NBP2-34270] is 200ug/ml. This format of the antibody also contains 0.05% BSA and 0.05% Azide as stabalizer and preservative respectively. So you can try this antibody at 1:100 - 1:200 dilution range, and based on your pilot results on your particular samples, you can go for lower or higher dilution for obtaining the best signal.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34675AF350
Alexa Fluor 405 NBP2-34675AF405
Alexa Fluor 488 NBP2-34675AF488
Alexa Fluor 532 NBP2-34675AF532
Alexa Fluor 594 NBP2-34675AF594
Alexa Fluor 647 NBP2-34675AF647
Alexa Fluor 700 NBP2-34675AF700
Alexa Fluor 750 NBP2-34675AF750
Allophycocyanin NBP2-34675APC
Biotin NBP2-34675B
DyLight 350 NBP2-34675UV
DyLight 405 NBP2-34675V
DyLight 488 NBP2-34675G
DyLight 550 NBP2-34675R
DyLight 594 NBP2-34675DL594
DyLight 650 NBP2-34675C
DyLight 680 NBP2-34675FR
DyLight 755 NBP2-34675IR
HRP NBP2-34675H
Janelia Fluor 549 NBP2-34675JF549
Janelia Fluor 646 NBP2-34675JF646
PE NBP2-34675PE

Additional Cytokeratin 14 Products

Bioinformatics Tool for Cytokeratin 14 Antibody (NBP2-34270)

Discover related pathways, diseases and genes to Cytokeratin 14 Antibody (NBP2-34270). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytokeratin 14 Antibody (NBP2-34270)

Discover more about diseases related to Cytokeratin 14 Antibody (NBP2-34270).
 

Pathways for Cytokeratin 14 Antibody (NBP2-34270)

View related products by pathway.

PTMs for Cytokeratin 14 Antibody (NBP2-34270)

Learn more about PTMs related to Cytokeratin 14 Antibody (NBP2-34270).
 

Research Areas for Cytokeratin 14 Antibody (NBP2-34270)

Find related products by research area.

Blogs on Cytokeratin 14

There are no specific blogs for Cytokeratin 14, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytokeratin 14 Antibody (LL002) and receive a gift card or discount.

Bioinformatics

Gene Symbol KRT14
Uniprot