p63/TP73L Antibody (CL3748) - Azide and BSA Free

Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.

Staining of human liver shows absence of immunoreactivity (negative control).

Staining of human prostate shows nuclear immunoreactivity in basal epithelial cells.

Staining of human lung cancer (adenocarcinoma) shows absence of immunoreactivity (negative control).

Staining of human prostate cancer shows absence of nuclear immunoreactivity in tumor cells, while it is present in the adjacent normal a basal epithelial cells.

Staining of human placenta shows nuclear positivity in a subset of cells.

Analysis in human cell line RT-4 and human cell line U-251 MG.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL3748
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

p63/TP73L Antibody (CL3748) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of Q9H3D4, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for p63/TP73L Antibody (CL3748) - Azide and BSA Free

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• p73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Background

The p63 gene, a homologue of the tumor suppressor p53, is highly expressed in the basal or progenitor layers of many epithelial tissues. P63 shows remarkable structural similarity to p53 and to the related p73 gene. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP63