PHD4/HIF Prolyl Hydroxylase 4 Antibody

Images

 
Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody used ...read more
Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Analysis of human PHD4, using NB100-295. Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.
Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4. Antibody: Affinity purified rabbit anti-PHD4 ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu(-)Species Glossary
Applications WB, EM, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

PHD4/HIF Prolyl Hydroxylase 4 Antibody Summary

Immunogen
The epitope recognized by this antibody maps to a region between residue 450 and the C-terminus (residue 502) of human PHD4/HIF Prolyl Hydroxylase 4 using the numbering given in entry NP_808808.1 (GeneID 54681).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P4HTM
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2500
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
Application Notes
This PHD4/HIF Prolyl Hydroxylase 4 antibody is useful for Western Blot where a band is seen at ~50 kDa. Use in Immunocytochemistry/immunofluorescence, Immunohistochemistry-Paraffin and Electron Microscopy reported in scientific literature (PMID 17003483) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
PHD4/HIF Prolyl Hydroxylase 4 Lysate (NBL1-14344)
Publications
Read Publications using
NB100-295 in the following applications:

  • EM
    1 publication
  • 1 publication
  • 1 publication
  • WB
    2 publications

Reactivity Notes

Does not appear to work in Mouse. Rat reactivity reported in scientific literature (PMID: 17003483)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PHD4/HIF Prolyl Hydroxylase 4 Antibody

  • EC 1.14.11.-
  • EGLN4
  • HIFPH4
  • HIF-PH4
  • HIF-prolyl hydroxylase 4
  • HPH-4
  • hypoxia-inducible factor prolyl 4-hydroxylase
  • Hypoxia-inducible factor prolyl hydroxylase 4
  • P4H with transmembrane domain
  • P4H-TMFLJ20262
  • PH4 hypoxia inducible factor prolyl 4 hydroxylase
  • PH-4
  • PHD4
  • proline 4-hydroxylase
  • prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
  • Prolyl hydroxlase domain-containing 4
  • transmembrane prolyl 4-hydroxylase

Background

HIF prolyl 4-hydroxylases (HIF-P4Hs or PHDs) are proyl hydroxylase domain-containing enzymes (PHD1, PHD2, PHD3, and P4H-TM) that mediate key physiological responses to hypoxia by modulating HIF1 alpha levels. PHD member proteins have a wide but varied tissue expression and PHD4/HIF Prolyl Hydroxylase 4 (P4H-TM) is a novel member of this family that localize to ER membrane as single-pass type II membrane protein with its catalytic site inside the lumen. P4H-TM is expressed mainly in pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland tissues and gets induced upon hypoxic trigger. P4H-TM catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins and hydroxylates HIF1A at 'Pro-402'/'Pro-564' sites. P4H-TM has been proposed to function as a cellular oxygen sensor and, under normoxic conditions, it targets HIF through hydroxylation for proteasomal degradation via VHL/von Hippel-Lindau ubiquitination complex. Ectopic over-expression of P4H-TM in cultured neuroblastoma cells was found to reduce HIF-1alpha ODDD reporter levels, whereas its knockdown increased HIF-1alpha protein levels. HIF-P4Hs plays a critical role in several pathologies such as myocardial infarction, stroke, peripheral vascular disease, inflammation, diabetes, carcinogenesis and severe anemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PHD4/HIF Prolyl Hydroxylase 4 Antibody (NB100-295)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PHD4/HIF Prolyl Hydroxylase 4 Antibody (NB100-295) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-295AF405
Alexa Fluor 488 Labeled NB100-295AF488
Alexa Fluor 647 Labeled NB100-295AF647
Alexa Fluor 700 Labeled NB100-295AF700
Biotin Labeled NB100-295B
DyLight 405 Labeled NB100-295V
DyLight 488 Labeled NB100-295G
DyLight 550 Labeled NB100-295R
DyLight 650 Labeled NB100-295C
FITC Labeled NB100-295F
HRP Labeled NB100-295H

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Bioinformatics

Gene Symbol P4HTM
Entrez
Uniprot