P4HA2 Antibody (CL0351) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Analysis in human epididymis and skeletal muscle tissues using NBP3-43972 antibody. Corresponding P4HA2 RNA-seq data are presented for the same tissues.

Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Staining of human epididymis shows moderate to strong cytoplasmic positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-P4HA2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0351
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

P4HA2 Antibody (CL0351) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse P4HA2 Antibody (CL0351) - Azide and BSA Free (NBP2-52922) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of O15460, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: P4HA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for P4HA2 Antibody (CL0351) - Azide and BSA Free

• 2-oxoglutarate-4-dioxygenase subunit alpha-2
• 4-PH alpha 2
• 4-PH alpha-2
• alpha polypeptide II
• collagen prolyl 4-hydroxylase alpha(II)
• C-P4Halpha(II)
• EC 1.14.11.2
• Procollagen-proline
• procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
• prolyl 4-hydroxylase subunit alpha-2
• prolyl 4-hydroxylase, alpha polypeptide II

Background

P4HA2 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P4HA2