BAI1 Antibody

Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.

Immunocytochemistry/Immunofluorescence: BAI1 Antibody [NB110-81586] - BAI1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586] - A. Staining of mouse brain using NB110-81586. B. Blocking of staining using control peptide, NB110-81586PEP.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

BAI1 Antibody Summary

• Immunogen: Synthetic peptide made to a C-terminal portion of the human BAI1 protein (within residues 1500-1584). [Swiss-Prot# O14514]
• Localization: Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADGRB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000-1:3000
  • Immunohistochemistry-Paraffin 1:1000-1:3000
• Application Notes: This BAI1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-paraffin embedded sections. Western Blot was reported in scientific literature.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BAI1 Antibody

• BAI1
• brain-specific angiogenesis inhibitor 1
• FLJ41988
• GDAIF

Background

BAI1 (brain-specific angiogenesis inhibitor 1) belongs to GPCR2 family or so-called adhesion-type family of 7-transmembrane receptors, and an extracellular fragment of this receptor was originally discovered to inhibit neovascularization in brain tumor model. BAI1 is a multi-pass membrane protein localized in cell membranes and is specifically expressed in brain with reduced or no expression in certain glioblastoma cell lines/cancer tissues and astrocytes. BAI1 is expressed on macrophages also, where it acts as a receptor for clearance of apoptotic cells via binding to surface phosphatidylserine of apoptotic cells through its type I TSRs followed by triggering their engulfment via an ELMO1/DOCK180/Rac1-mediated signaling pathway. BAI1 interacts with several proteins including MAGI1, MAGI3, BAIAP2, PHYHIP, ELMO1 and DOCK1. BAI1 plays a unique function in binding and internalization of Gram-negative bacteria through recognition of bacterial LPS via BAI1-TSRs. It has been proposed as a potent inhibitor of brain angiogenesis, a mediator of p53 signal in suppression of glioblastoma, and functions in cell adhesion as well as signal transduction in brain. BAI1 also regulates phagocytosis, synaptogenesis, inhibition of tumor growth and angiogenesis via processing of its extracellular domain into secreted vasculostatins, and it is emerging as a therapeutics target for diseases such as cancer, stroke, and schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BAI1 Antibody (NB110-81586)(2)

Publications using NB110-81586

• Zhu D, Osuka S, Zhang Z et al. BAI1 Suppresses Medulloblastoma Formation by Protecting p53 from Mdm2-Mediated Degradation Cancer Cell Jun 11 2018 [PMID: 29894688] (Human, Mouse)
• Hochreiter-Hufford AE, Lee CS, Kinchen JM et al. Phosphatidylserine receptor BAI1 and apoptotic cells as new promoters of myoblast fusion. Nature 2013 May 9 [PMID: 23615608] (WB, Mouse)

Reviews for BAI1 Antibody (NB110-81586) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Anonymous ICC Mouse 03/04/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse cerebellum
• Species: Mouse

Blocking

• Blocking Details: 5% BSA, RT 1 hour

Details

• Detection Notes: ABC standard method, use DAB as substrate.

Comments

• Comments: The Purkinje cells (arrows) show relatively strong positive staining, but the molecular layer and granular layer seem to have some non-specific staining.

reviewed by: Anonymous WB Mouse 12/05/2014

Summary

• Application: Western Blot
• Sample Tested: Mouse Brain Tissue
• Species: Mouse

Blocking

• Blocking Details: 5% milk, room temperature, 1 hour

Primary Anitbody

• Dilution Ratio: 1:1000 dilution in 0.1% TBST, 16 hrs at 4 degree

Secondary Antibody

• Secondary Description: HRP-conjugated goat anti-rabbit IgG
• Secondary Manufacturer Cat#: Thermo
• Secondary Concentration: 1:10,000 dilution

Details

• Detection Notes: ECL, 10 seconds, KO mice as negative controls

Bioinformatics

• Gene Symbol: ADGRB1