BAI1 Antibody

Images

 
Western Blot: BAI1 Antibody [NB110-81586] - analysis of BAI1 in mouse brain tissue using anti-BAI1 antibody. KO mice was used as negative controls (right lane). Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: BAI1 Antibody [NB110-81586] - BAI1 antibody was tested in SH-SY-5Y cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: BAI1 Antibody [NB110-81586] - A. Staining of mouse brain using NB110-81586. B. Blocking of staining using control peptide, NB110-81586PEP.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

BAI1 Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the human BAI1 protein (within residues 1500-1584). [Swiss-Prot# O14514]
Localization
Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:1000-1:3000
  • Immunohistochemistry-Paraffin 1:1000-1:3000
Application Notes
This BAI1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-paraffin embedded sections. Western Blot was reported in scientific literature.
Control Peptide
BAI1 Peptide (NB110-81586PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB110-81586 in the following applications:

Publications
Read Publication using
NB110-81586 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BAI1 Antibody

  • BAI1
  • brain-specific angiogenesis inhibitor 1
  • FLJ41988
  • GDAIF

Background

BAI1 (brain-specific angiogenesis inhibitor 1) belongs to GPCR2 family or so-called adhesion-type family of 7-transmembrane receptors, and an extracellular fragment of this receptor was originally discovered to inhibit neovascularization in brain tumor model. BAI1 is a multi-pass membrane protein localized in cell membranes and is specifically expressed in brain with reduced or no expression in certain glioblastoma cell lines/cancer tissues and astrocytes. BAI1 is expressed on macrophages also, where it acts as a receptor for clearance of apoptotic cells via binding to surface phosphatidylserine of apoptotic cells through its type I TSRs followed by triggering their engulfment via an ELMO1/DOCK180/Rac1-mediated signaling pathway. BAI1 interacts with several proteins including MAGI1, MAGI3, BAIAP2, PHYHIP, ELMO1 and DOCK1. BAI1 plays a unique function in binding and internalization of Gram-negative bacteria through recognition of bacterial LPS via BAI1-TSRs. It has been proposed as a potent inhibitor of brain angiogenesis, a mediator of p53 signal in suppression of glioblastoma, and functions in cell adhesion as well as signal transduction in brain. BAI1 also regulates phagocytosis, synaptogenesis, inhibition of tumor growth and angiogenesis via processing of its extracellular domain into secreted vasculostatins, and it is emerging as a therapeutics target for diseases such as cancer, stroke, and schizophrenia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BAI1 Antibody (NB110-81586)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Reviews for BAI1 Antibody (NB110-81586) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB110-81586:
Filter by Applications
ICC
(1)
WB
(1)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry BAI1 NB110-81586
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reviewed by:
Anonymous
ICC Mouse 03/04/2016
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Summary

ApplicationImmunocytochemistry
Sample Testedmouse cerebellum
SpeciesMouse

Blocking

Blocking Details5% BSA, RT 1 hour

Details

Detection NotesABC standard method, use DAB as substrate.

Comments

CommentsThe Purkinje cells (arrows) show relatively strong positive staining, but the molecular layer and granular layer seem to have some non-specific staining.
Western Blot BAI1 NB110-81586
Enlarge
reviewed by:
Anonymous
WB Mouse 12/05/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse Brain Tissue
SpeciesMouse

Blocking

Blocking Details5% milk, room temperature, 1 hour

Primary Anitbody

Dilution Ratio1:1000 dilution in 0.1% TBST, 16 hrs at 4 degree

Secondary Antibody

Secondary DescriptionHRP-conjugated goat anti-rabbit IgG
Secondary Manufacturer Cat#Thermo
Secondary Concentration1:10,000 dilution

Details

Detection NotesECL, 10 seconds, KO mice as negative controls

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BAI1 Antibody (NB110-81586) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-81586AF405
Alexa Fluor 488 Labeled NB110-81586AF488
Alexa Fluor 647 Labeled NB110-81586AF647
Alexa Fluor 700 Labeled NB110-81586AF700
Biotin Labeled NB110-81586B
DyLight 405 Labeled NB110-81586V
DyLight 488 Labeled NB110-81586G
DyLight 550 Labeled NB110-81586R
DyLight 650 Labeled NB110-81586C
FITC Labeled NB110-81586F
HRP Labeled NB110-81586H

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Blogs on BAI1

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
03/04/2016
Application: ICC
Species: Mouse

Anonymous
12/05/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol BAI1
Entrez