Otx2 Antibody [Biotin]

Otx2 was detected in immersion fixed BG01V human embryonic stem cells differentiated to ectoderm using Goat Anti-Human Otx2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Cells were co-stained using anti-Tubulin (Catalog # NB600-506, Novus Biologicals) and NorthernLights™ 493-conjugated AntI-Rat IgG Secondary Antibody (green; Catalog # NL015). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Otx2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Otx2
  Met1-Leu289
  Accession # P32243
• Specificity: Detects human Otx2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OTX2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody [Biotin]

• homeobox protein OTX2
• MCOPS5
• MGC45000
• orthodenticle homeobox 2
• orthodenticle homolog 2 (Drosophila)
• Orthodenticle homolog 2
• Otx2

Background

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otx2 Antibody (BAF1979)(20)

Publications using BAF1979

• C Finkbeiner, I Ortuño-Liz, A Sridhar, M Hooper, S Petter, TA Reh Single-cell ATAC-seq of fetal human retina and stem-cell-derived retinal organoids shows changing chromatin landscapes during cell fate acquisition Cell Reports, 2022;38(4):110294. 2022-01-01 [PMID: 35081356] (IHC, Human)
• L Todd, MJ Hooper, AK Haugan, C Finkbeiner, N Jorstad, N Radulovich, CK Wong, PC Donaldson, W Jenkins, Q Chen, F Rieke, TA Reh Efficient stimulation of retinal regeneration from M�ller glia in adult mice using combinations of proneural bHLH transcription factors Cell Reports, 2021;37(3):109857. 2021-01-01 [PMID: 34686336] (IF, Mouse)
• L Todd, C Finkbeiner, CK Wong, MJ Hooper, TA Reh Microglia Suppress Ascl1-Induced Retinal Regeneration in Mice Cell Reports, 2020;33(11):108507. 2020-01-01 [PMID: 33326790] (Flow Cytometry, Transgenic Mouse)
• NL Jorstad, MS Wilken, L Todd, C Finkbeiner, P Nakamura, N Radulovich, MJ Hooper, A Chitsazan, BA Wilkerson, F Rieke, TA Reh STAT Signaling Modifies Ascl1 Chromatin Binding and Limits Neural Regeneration from Muller Glia in Adult Mouse Retina Cell Rep, 2020;30(7):2195-2208.e5. 2020-01-01 [PMID: 32075759] (IHC, Mouse)
• A Sridhar, A Hoshino, CR Finkbeiner, A Chitsazan, L Dai, AK Haugan, KM Eschenbach, DL Jackson, C Trapnell, O Bermingham, I Glass, TA Reh Single-Cell Transcriptomic Comparison of Human Fetal Retina, hPSC-Derived Retinal Organoids, and Long-Term Retinal Cultures Cell Rep, 2020;30(5):1644-1659.e4. 2020-01-01 [PMID: 32023475] (IHC, Human)
• A Hoshino, S Horvath, A Sridhar, A Chitsazan, TA Reh Synchrony and asynchrony between an epigenetic clock and developmental timing Sci Rep, 2019;9(1):3770. 2019-01-01 [PMID: 30842553] (IHC, Human)
• CL Fairchild, K Hino, JS Han, AM Miltner, GP Allina, CE Brown, ME Burns, A La Torre, S Simo RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion Development, 2018;0(0):. 2018-01-01 [PMID: 29361558] (IHC, Mouse)
• TS Mills, T Eliseeva, SM Bersie, G Randazzo, J Nahreini, KU Park, JA Brzezinski Combinatorial regulation of a Blimp1 (Prdm1) enhancer in the mouse retina PLoS ONE, 2017;12(8):e0176905. 2017-01-01 [PMID: 28829770] (Western Blot, Mouse)
• TP Sharma, LA Wiley, SS Whitmore, KR Anfinson, CM Cranston, DJ Oppedal, HT Daggett, RF Mullins, BA Tucker, EM Stone Patient-specific induced pluripotent stem cells to evaluate the pathophysiology of TRNT1-associated Retinitis pigmentosa Stem Cell Res, 2017;21(0):58-70. 2017-01-01 [PMID: 28390992] (ICC, Human)
• A Moshiri, D Humpal, BC Leonard, DM Imai, A Tham, L Bower, D Clary, TM Glaser, KC Lloyd, CJ Murphy Arap1 Deficiency Causes Photoreceptor Degeneration in Mice Invest. Ophthalmol. Vis. Sci, 2017;58(3):1709-1718. 2017-01-01 [PMID: 28324111] (IHC, Mouse)
• KU Park, G Randazzo, KL Jones, JA Brzezinski Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina Invest. Ophthalmol. Vis. Sci, 2017;58(2):1137-1150. 2017-01-01 [PMID: 28199486] (IHC-Fr, Mouse)
• cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness Sci Rep, 2016;6(0):30742. 2016-01-01 [PMID: 27471043] (IHC-Fr, Mouse)
• La Torre A, Hoshino A, Cavanaugh C, Ware C, Reh T The GIPC1-Akt1 Pathway Is Required for the Specification of the Eye Field in Mouse Embryonic Stem Cells. Stem Cells, 2015;33(9):2674-85. 2015-01-01 [PMID: 26013465] (IHC, Mouse)
• Ueki Y, Wilken M, Cox K, Chipman L, Bermingham-McDonogh O, Reh T A transient wave of BMP signaling in the retina is necessary for Muller glial differentiation. Development, 2015;142(3):533-43. 2015-01-01 [PMID: 25605781] (IHC, Mouse)
• Klimova , Lucie, Kozmik , Zbynek Stage-dependent requirement of neuroretinal Pax6 for lens and retina development. Development, 2014;141(6):1292-302. 2014-01-01 [PMID: 24523460] (IHC-Fr, Mouse)
• Brzezinski J, Uoon Park K, Reh T Blimp1 (Prdm1) prevents re-specification of photoreceptors into retinal bipolar cells by restricting competence. Dev Biol, 2013;384(2):194-204. 2013-01-01 [PMID: 24125957] (Immunoprecipitation, Mouse)
• Tucker B, Anfinson K, Mullins R, Stone E, Young M Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation. Stem Cells Transl Med, 2013;2(1):16-24. 2013-01-01 [PMID: 23283489] (ICC, Human)
• Achim K, Peltopuro P, Lahti L Distinct developmental origins and regulatory mechanisms for GABAergic neurons associated with dopaminergic nuclei in the ventral mesodiencephalic region. Development, 2012;139(13):2360-70. 2012-01-01 [PMID: 22627282] (IHC-P, Mouse)
• Brzezinski JA, Lamba DA, Reh TA Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development. Development, 2010;137(4):619-29. 2010-01-01 [PMID: 20110327] (IHC-Fr, Mouse)
• Adamson DC, Shi Q, Wortham M OTX2 is critical for the maintenance and progression of Shh-independent medulloblastomas. Cancer Res., 2010;70(1):181-91. 2010-01-01 [PMID: 20028867] (Western Blot, Human)

Bioinformatics

• Gene Symbol: OTX2
• Uniprot:
  • P32243()