GBX2 Antibody

Images

 
Western Blot: GBX2 Antibody [NB100-55393] - (0.1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: GBX2 Antibody [NB100-55393] - Gbx2 Antibody [NB100-55393] - (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GBX2 Antibody Summary

Immunogen
Peptide with sequence C-QGKDESKVEDDPK corresponding to internal region according to NP_001476.2.
Epitope
QGKDESKVEDDPK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GBX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 4 - 8 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 37 kDa band observed in lysates of T-cell line Jurkat (calculated MW of 37.3 kDa band according to NP_001476.2). IHC-P: Human placenta shows nuclear staining of selected trophoblasts.
IHC: In paraffin embedded Human Placenta shows nuclear staining of selected trophoblasts.
Control
Jurkat Lysate (NB800-PC2)
Publications
Read Publications using
NB100-55393 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GBX2 Antibody

  • Gastrulation and brain-specific homeobox protein 2
  • gastrulation brain homeo box 2
  • gastrulation brain homeobox 2
  • GBX2
  • homeobox protein GBX-2

Background

GBX2 (Gastrulation brain homeobox 2) is a homeobox protein which is involved in enbryonic cell pluripotency and differentiation. GBX2 is expressed in all three embryonic germ layers of the late gastrula/early neurula. Gbx2 is overexpressed in a panel of human prostatic cancer cell lines (TSU-pr1, PC3, DU145, LNCaP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GBX2 Antibody (NB100-55393)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GBX2
Entrez
Uniprot