RAX Antibody

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Western Blot: RAX Antibody [NB100-56467] - analysis of Rax in the 1) absence and 2) presence of immunizing peptide in Jurkat cell lysate using this antibody. 5 ug/ml.

Product Details

Summary
Reactivity Hu, Ca, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RAX Antibody Summary

Immunogen
A portion of amino acids 300-350 of human Rax was used as the immunogen.
Specificity
The amino acid sequence used as immunogen has 100% identity in human, dog, and monkey.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Control
Jurkat Lysate (NB800-PC2)

Reactivity Notes

Reacts with Canine, Human and New World Monkey. 85% sequence identity with chicken and 78% with zebrafish, mouse and rat proteins.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAX Antibody

  • MCOP3
  • retina and anterior neural fold homeobox
  • retinal homeobox protein Rx
  • RXRetina and anterior neural fold homeobox protein

Background

Principal Names: Retinal and anterior neural fold Homeobox (Rax); Retinal Homeobox protein Rx; Official Gene Symbol: RAX Gen Bank Accession Number: NP_038463 Gene ID: 30062 (Human) Gene Map Locus: 18q21.32 (Human) Rax, a novel transcription factor, is a human homolog of Drosophila Rx protein. It is a significant member of Rx protein family and consists of a highly-conserved N-terminal DNA-binding paired-like Homeodomain. It interacts with PCE-1 and is one of the earliest expressed proteins stimulating establishment and proliferation of retinal progenitor cells. Its expression is essential in determining the fate of normal eye development. Irregular expression of Rax has profound effects on eye morphogenesis. Mutations in Rax gene have been detected in clinical conditions like Anophthalmia and Sclerocornea.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RAX Antibody (NB100-56467). (Showing 1 - 1 of 1 FAQ).

  1. Does this product work in zebrafish?
    • NB100-56467 has been validated in human, canine, and primate. We have not yet tested this in zebrafish. The immunogen used (from human RAX) shares a 78% homology with zebrafish, so there may be some potential for cross reaction. However, we do not have any testing information nor can we guarantee performance in zebrafish.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NB100-56467

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Bioinformatics

Gene Symbol RAX
Entrez
Uniprot