engrailed homeobox 2 Antibody

Images

 
Western Blot: engrailed homeobox 2 Antibody [NB100-2814] - Western blot analysis of Mouse Brain lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) ...read more
Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody [NB100-2814] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

engrailed homeobox 2 Antibody Summary

Immunogen
Peptide with sequence C-NHSTTAKEGKSDSE corresponding to C-Terminus according to NP_001418.2.
Predicted Species
Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:4000
Application Notes
Western blot: Approx 35kDa band observed in Mouse Brain lysates (calculated MW of f 33.8kDa according to Mouse NP_034264.1).
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publication using NB100-2814.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for engrailed homeobox 2 Antibody

  • AUTS10
  • EN2
  • engrailed homeobox 2
  • engrailed homolog 2
  • Engrailed2
  • Engrailed-2
  • Homeobox protein en-2
  • homeobox protein engrailed-2
  • hu-En-2

Background

EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for engrailed homeobox 2 Antibody (NB100-2814)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for engrailed homeobox 2 Antibody (NB100-2814) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol EN2
Entrez
Uniprot