Otx2 Antibody [Unconjugated]

Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Otx2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Otx2 was detected in immersion fixed paraffin-embedded sections of mouse embryo (14 d.p.c.) using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing nervous system. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of IMR‑32 human neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Otx2 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). (A) Dermal fibroblasts derived from patient with SLE were reprogrammed into iPSCs using Sendai virus vectors and three clones (#1, #2 and #3) were characterized. RT‐PCR confirms the loss of transgenes in hiPSCs‐SLE (lanes 1, 2 and 3), presence (lane 4) in infected fibroblasts (ipF‐SLE) and absence of Sendai viral transgenes in parental fibroblasts (pF‐SLE) (lane 5). Full‐length gels are presented in File S2. (B) The hiPSCs‐SLE colonies expressed alkaline phosphatase. Scale bar, 500 μm. (C) RT‐qPCR analysis of pluripotency genes OCT4, NANOG, SOX2 and REX1 was performed in fibroblasts and in hiPSCs derived from patient with SLE. All expression values are normalized to GAPDH and relative donor fibroblasts. Data are mean ± SEM and all statistical analysis was made between hiPSCs‐SLE clones and relative fibroblasts by Student's t test showing P‐values ≤ .05 in each comparison. (D) PluriTest assays combines novelty score (blue) on x‐axis and pluripotency score (red) on y‐axis. hiPSCs‐SLE localize in the red cloud suggesting the empirical distribution of pluripotent cells compared to non‐pluripotent blue cloud. (E) Representative images of M‐FISH staining show normal karyotypes of hiPSCs‐SLE clones. (F) Immunofluorescence analysis of pluripotent stem cell markers Nanog (green), Oct4 (red) and co‐staining with DAPI (blue) in hiPSCs‐SLE. Scale bar, 50 μm. (G) Representative images of floating and adherent EBs derived from hiPSCs‐SLE at differentiation day 8 and 18, respectively. Scale bar, 500 μm. (H) RT‐qPCR results confirm the capability of hiPSCs‐SLE to differentiate into all three germ layers. The expression levels of GATA4, HAND1 and PAX6 in EBs are relative to undifferentiated hiPSCs. All expression values are normalized to GAPDH and relative hiPSCs. Data are mean ± SEM and all statistical analysis was made between EBs‐SLE and relative hiPSCs‐SLE clones by Student's t test showing P‐values ≤ .05 in each comparison. (I) Immunostaining of Otx2 (ectoderm marker), Sox17 (endoderm marker), Brachyury (mesoderm marker) and co‐staining with DAPI (blue) in EBs derived from hiPSCs‐SLE. Scale bar, 50 μm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31536674), licensed under a CC-BY license. Not internally tested by R&D Systems.

Medaka-derived organoids show onset of retinal differentiation. (d) Optical sections showing expression of HuC/D (amacrine&ganglion cells), Otx2 (bipolar&photoreceptor cells),&Prox1 (horizontal cells) in day 4 organoid. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.

The short-term effects of Notch inhibition on RPC reporters. (d) Quantification of the %age of VSX2, Otx2&double marker-positive cells within the electroporated cell population from cell counting. Sectioned chick retinas electroporated ex vivo at E5 with the co-electroporation control CAG::Nuc beta gal with or without CAG::MAML-DN. The retinas cultured for 20 h&immunostained with VSX2 or Otx2 antibodies. The Shapiro–Wilk normality test was used to confirm the normal distribution. * signifies p < 0.05, *** signifies p < 0.001 with a two-tailed student’s t-test. Each point represents one biological replicate. The columns represent mean,&the error bars represent standard deviation. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34267251), licensed under a CC-BY license. Not internally tested by R&D Systems.

Targeted expression of MAML-DN to cone/HC restricted RPCs does not alter cone formation. (d) Flow cytometry quantification of the %age of Otx2, Lim1, AP2 alpha , OC1ECR22&ThrbCRM2 reporter-positive cells within the ThrbCRM1 lineage traced cell population. Dissociated chick retinal cells electroporated ex vivo at E5 with the co-electroporation control CAG::TdTomato, ThrbCRM1::PhiC31&its responder plasmid,&with or without CAG::MAML-DN. The retinas cultured for 2 days post-electroporation. The Shapiro–Wilk normality test was used to confirm the normal distribution. Mann–Whitney test was used to test significance in ThrbCRM2 quantification. A two-tailed student’s t-test was used to test significance in the rest of the quantifications. * signifies p < 0.05, ** signifies p < 0.01. Each point represents one biological replicate. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34267251), licensed under a CC-BY license. Not internally tested by R&D Systems.

Medaka primary embryonic pluripotent cells form optic vesicle-like structures. (f) Maximal projection of day 2 organoids&1 dpf embryo generated from Rx3::H2B-GFP transgenic or wild-type blastulae&stained with neural tissue-specific anti-Sox2 (n = 12/12)&anti-Otx2 (n = 10/10) antibodies, co-stained with anti-Rx2&DAPI nuclear stain. hpa, hours post-aggregation; dpf, days post-fertilization. Scale bars: 100 μm.Overview of complexity&variability of organoid morphology generated within one experiment.Overlay of fluorescence&bright-field images of retinal organoids on day 2 generated from the Rx3::H2B-GFP reporter line formed by aggregation of <1000 primary embryonic pluripotent cells stained with anti-GFP antibody. Scale bar: 100 μm.Examples of organoids generated by aggregation of <1000 cells forming one, two,&three optic vesicle-like structures.Fluorescence images, bright-field images,&their overlay of retinal organoids on day 2 generated from the Rx3::H2B-GFP reporter line formed by aggregation of <1000 primary embryonic pluripotent cells stained with anti-GFP antibody. Scale bar: 100 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.

Examples of organoids generated by aggregation of <1000 cells forming one, two, and three optic vesicle-like structures.Fluorescence images, bright-field images, and their overlay of retinal organoids on day 2 generated from the Rx3::H2B-GFP reporter line formed by aggregation of <1000 primary embryonic pluripotent cells stained with anti-GFP antibody. Scale bar: 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.

Medaka primary embryonic pluripotent cells form optic vesicle-like structures. (e) Bright-field&fluorescent images of day 2 Rx3::H2B-GFP organoids stained with anti-GFP antibody. Optical sections of an organoid (day 2) (n = 9/10)&an embryo (1 dpf) stained with anti-Rx2&anti-Otx2 antibodies. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34252023), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Otx2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Otx2
  Met1-Leu289
  Accession # P32243
• Specificity: Detects human Otx2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OTX2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Otx2 Antibody [Unconjugated]

• homeobox protein OTX2
• MCOPS5
• MGC45000
• orthodenticle homeobox 2
• orthodenticle homolog 2 (Drosophila)
• Orthodenticle homolog 2
• Otx2

Background

Otx2 is a member of the bicoid subfamily of homeodomain-containing transcription factors. It may play a role in brain and sensory organ development (1). Isoform a differs from isoform b by having an eight amino acid residue insertion between P32 and A33 of Otx2b.

1. Nagao, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Otx2 Antibody (AF1979)(181)

Publications using AF1979

• Chen, Y;Jiang, M;Li, L;Yang, S;Liu, Z;Lin, S;Wang, W;Li, J;Chen, F;Hou, Q;Ma, X;Hou, L; Absent in melanoma 2: a potent suppressor of retinal pigment epithelial-mesenchymal transition and experimental proliferative vitreoretinopathy Cell death & disease 2025-01-27 [PMID: 39870644] (Immunohistochemistry, Mouse)
• Atash, A;Cramer, MJ;Mees, B;Doevendans, PA;Sluijter, JPG;Stillitano, F; Generation and characterization of novel induced pluripotent stem cell (iPSC) lines derived from three symptomatic carriers of a pathogenic MYH11 variant and two non-carrier relatives Stem cell research 2024-12-12 [PMID: 39705883] (Immunocytochemistry, Human)
• Tran, HD;Denman, CR;Shin, MK;Jeon, D;Kuhn, B;Jo, J; Establishment of TH-EGFP human embryonic stem cell line for specific labeling of dopaminergic neurons Stem cell research 2024-11-17 [PMID: 39577309] (Immunocytochemistry, Human)
• Hofstetter, KS;Haas, PM;Kuntz, JP;Zheng, Y;Fuhrmann, S; Loss of Cdc42 causes abnormal optic cup morphogenesis and microphthalmia in mouse bioRxiv : the preprint server for biology 2024-10-22 [PMID: 39484575] (Immunohistochemistry, Transgenic Mouse)
• Afting, C;Walther, T;Drozdowski, OM;Schlagheck, C;Schwarz, US;Wittbrodt, J;Göpfrich, K; DNA microbeads for spatio-temporally controlled morphogen release within organoids Nature nanotechnology 2024-09-09 [PMID: 39251862] (Immunocytochemistry, Human)
• Zhao, M;Wang, J;Liu, M;Xu, Y;Huang, J;Zhang, Y;He, J;Gu, A;Liu, M;Liu, X; KIF1A, R1457Q, and P1688L Mutations Induce Protein Abnormal Aggregation and Autophagy Impairment in iPSC-Derived Motor Neurons Biomedicines 2024-07-30 [PMID: 39200158] (Immunocytochemistry, Human)
• Sun, J;Ren, L;Canel Rivero, G;Xu, L;Ladabaum, U;C Wu, J; Generation of two induced pluripotent stem cell lines from patients with Li-Fraumeni Syndrome carrying TP53 mutation Stem cell research 2024-08-03 [PMID: 39133963] (Immunocytochemistry, Human)
• Qin, H;Yu, Y;Ye, S;Alonzo, M;Garg, V;Zhao, MT; Generation of an induced pluripotent stem cell line (NCHi016-A) from a 5-year-old female with pulmonary atresia with intact ventricular septum and one-and-half ventricle palliation Stem cell research 2024-08-05 [PMID: 39126918] (Immunocytochemistry, Human)
• Bianchini, L;Sieber, L;Hammad, R;Schäfer, R;Kutscher, LM; Generation of two isogenic patient-derived human-induced pluripotent stem cell clones with 6q27 deletion Stem cell research 2024-10-01 [PMID: 39106599] (Immunocytochemistry, Human)
• Tang, P;Keshi, E;Wilken, S;Wutsdorff, L;Mougnekabol, J;Pratschke, J;Sauer, IM;Haep, N; Generation of an induced pluripotent stem cell (iPSC) line (EXSURGi001-A) from a patient homozygous for the p.Ala165Thr mutation in the MTARC1 gene Stem cell research 2024-10-01 [PMID: 39094505] (Immunohistochemistry, Human)
• Schumacher, S;Fernkorn, M;Marten, M;Chen, R;Kim, YS;Bedzhov, I;Schröter, C; Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation Nature communications 2024-06-13 [PMID: 38871742] (Immunohistochemistry, Human)
• Dai, Y;Zhu, W;Flores Banuelos, AG;Li, J;Mukherjee, S;Algaze, C;Wu, JC; Generation of two induced pluripotent stem cell lines from patients with Williams syndrome Stem cell research 2024-08-01 [PMID: 38861775] (Immunocytochemistry, Human)
• Wang, J;Bering, J;Alonzo, M;Ye, S;Texter, K;Garg, V;Zhao, MT; Generation of iPSC line NCHi015-A from a patient with truncus arteriosus carrying heterozygous variants in KMT2D and NOTCH1 Stem cell research 2024-05-29 [PMID: 38833814] (Immunocytochemistry, Human)
• Yarkova, ES;Grigor'eva, EV;Medvedev, SP;Tarasevich, DA;Pavlova, SV;Valetdinova, KR;Minina, JM;Zakian, SM;Malakhova, AA; Detection of ER Stress in iPSC-Derived Neurons Carrying the p.N370S Mutation in the GBA1 Gene Biomedicines 2024-03-27 [PMID: 38672099] (Immunocytochemistry, Human)
• Kairišs, K;Sokolova, N;Zilova, L;Schlagheck, C;Reinhardt, R;Baumbach, T;Faragó, T;van de Kamp, T;Wittbrodt, J;Weinhardt, V; Visualisation of gene expression within the context of tissues using an X-ray computed tomography-based multimodal approach Scientific reports 2024-04-12 [PMID: 38609416] (Immunohistochemistry, Fish - Oryzias latipes (Medaka or Japanese Rice Fish))
• Weatherbee, BAT;Weberling, A;Gantner, CW;Iwamoto-Stohl, LK;Barnikel, Z;Barrie, A;Campbell, A;Cunningham, P;Drezet, C;Efstathiou, P;Fishel, S;Vindel, SG;Lockwood, M;Oakley, R;Pretty, C;Chowdhury, N;Richardson, L;Mania, A;Weavers, L;Christie, L;Elder, K;Snell, P;Zernicka-Goetz, M; Distinct pathways drive anterior hypoblast specification in the implanting human embryo Nature cell biology 2024-03-01 [PMID: 38443567] (Immunohistochemistry, Human, Mouse)
• Daya, NM;Döring, K;Zhuge, H;Volke, L;Stab, V;Dietz, J;Athamneh, M;Roos, A;Zaehres, H;Güttsches, AK;Mavrommatis, L;Vorgerd, M; Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C Stem cell research 2024-01-27 [PMID: 38309149] (Flow Cytometry, Immunocytochemistry, Human)
• Yan, Y;Li, X;Gao, Y;Mathivanan, S;Kong, L;Tao, Y;Dong, Y;Li, X;Bhattacharyya, A;Zhao, X;Zhang, SC; 3D bioprinting of human neural tissues with functional connectivity Cell Stem Cell 2024-02-01 [PMID: 38306994] (Immunocytochemistry, Human)
• Tripathi, D;Manhas, A;Noishiki, C;Wu, D;Adkar, S;Sallam, K;Fukaya, E;Leeper, NJ;Sayed, N; Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC) Stem cell research 2023-12-19 [PMID: 38199067] (IHC, Human)
• Park, S;Park, CW;Eom, JH;Jo, MY;Hur, HJ;Choi, SK;Lee, JS;Nam, ST;Jo, KS;Oh, YW;Lee, J;Kim, S;Kim, DH;Park, CY;Kim, SJ;Lee, HY;Cho, MS;Kim, DS;Kim, DW; Preclinical and dose-ranging assessment of hESC-derived dopaminergic progenitors for a clinical trial on Parkinson's disease Cell stem cell 2023-12-07 [PMID: 38086390] (Immunocytochemistry, Human)
• Maimaitili, M;Chen, M;Febbraro, F;Ucuncu, E;Kelly, R;Niclis, JC;Christiansen, JR;Mermet-Joret, N;Niculescu, D;Lauritsen, J;Iannielli, A;Klæstrup, IH;Jensen, UB;Qvist, P;Nabavi, S;Broccoli, V;Nykjær, A;Romero-Ramos, M;Denham, M; Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells Nature communications 2023-12-05 [PMID: 38052784] (Immunohistochemistry, Immunocytochemistry, Human, Rat)
• Driver, K;Vo, C;Scriba, CK;Saker, S;Larmonier, T;Malfatti, E;Romero, NB;Ravenscroft, G;Laing, NG;Taylor, RL;Clayton, JS; Generation of two induced pluripotent stem cell lines from a 33-year-old central core disease patient with a heterozygous dominant c.14145_14156delCTACTGGGACA (p.Asn4715_Asp4718del) deletion in the RYR1 gene Stem cell research 2023-11-22 [PMID: 38029555] (ICC, Human)
• Tao, Y;Li, X;Dong, Q;Kong, L;Petersen, AJ;Yan, Y;Xu, K;Zima, S;Li, Y;Schmidt, DK;Ayala, M;Mathivanan, S;Sousa, AMM;Chang, Q;Zhang, SC; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells Nature biotechnology 2023-11-16 [PMID: 37974010] (IHC, Flow Cytometry, Mouse)
• Meyer, K;Lammers, NC;Bugaj, LJ;Garcia, HG;Weiner, OD; Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation Nature communications 2023-10-30 [PMID: 37903793] (ICC, Mouse)
• Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F; Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative Stem cell research 2023-09-21 [PMID: 37748331] (ICC, Human)
• Maura K. Schwartz, Shibi Likhite, Tatyana A. Vetter, Megan C. Baird, Vicki McGovern, Andrea Sierra Delgado et al. In-depth comparison of Anc80L65 and AAV9 retinal targeting and characterization of cross-reactivity to multiple AAV serotypes in humans Molecular Therapy - Methods & Clinical Development 2023-09-14 [PMID: 37746244]
• Liu, W;Zeng, W;Kong, X;Htet, M;Yu, R;Wheeler, M;Day, JW;Wu, JC; Generation of two induced pluripotent stem cell lines from Duchenne muscular dystrophy patients Stem cell research 2023-09-18 [PMID: 37740996] (ICC, Flow Cytometry, Human)
• Kenyu Iwatsuki, Mami Oikawa, Hisato Kobayashi, Christopher A. Penfold, Makoto Sanbo, Takuya Yamamoto et al. Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells Cell Reports Methods 2023-08-28 [PMID: 37671016] (Immunocytochemistry, Rat)
• Jourdon, A;Wu, F;Mariani, J;Capauto, D;Norton, S;Tomasini, L;Amiri, A;Suvakov, M;Schreiner, JD;Jang, Y;Panda, A;Nguyen, CK;Cummings, EM;Han, G;Powell, K;Szekely, A;McPartland, JC;Pelphrey, K;Chawarska, K;Ventola, P;Abyzov, A;Vaccarino, FM; Modeling idiopathic autism in forebrain organoids reveals an imbalance of excitatory cortical neuron subtypes during early neurogenesis Nature neuroscience 2023-08-10 [PMID: 37563294] (IHC, Human)
• Cunningham, D;Stanberry, I;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B; Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene Stem cell research 2023-08-01 [PMID: 37549562] (ICC, Human)
• Mehmet Girgin, Nicolas Broguiere, Lorenzo Mattolini, Matthias Lutolf A New Approach to Generate Gastruloids to Develop Anterior Neural Tissues BIO-PROTOCOL 2023-07-20 [PMID: 37497450]
• Lin Feng, Da Li, Yao Tian, Chengshun Zhao, Yun Sun, Xiaolong Kou et al. One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo Neural Regeneration Research 2024-02-01 [PMID: 37488911]
• Muwan Chen, Jonathan Christos Niclis, Mark Denham Protocol for generating reproducible miniaturized controlled midbrain organoids STAR Protocols 2023-09-01 [PMID: 37481727]
• Yu Y, Alonzo M, Ye S et al. Generation of an induced pluripotent stem cell line (NCHi010-A) from a 6-year-old female with Down syndrome and without congenital heart disease Stem Cell Research 2023-09-01 [PMID: 37392705]
• Kaneyasu Nishimura, Emilía Sif Ásgrímsdóttir, Shanzheng Yang, Ernest Arenas A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons STAR Protocols 2023-09-01 [PMID: 37310863]
• Zeng B, Liu Z, Lu Y et al. The single-cell and spatial transcriptional landscape of human gastrulation and early brain development Cell stem cell 2023-05-12 [PMID: 37192616]
• W Zeng, X Kong, C Alamana, Y Liu, J Guzman, PD Pang, JW Day, JC Wu Generation of two induced pluripotent stem cell lines from spinal muscular atrophy type 1 patients carrying no functional copies of SMN1 gene Stem Cell Research, 2023-04-17;69(0):103095. 2023-04-17 [PMID: 37087898] (ICC, Human)
• J Li, Q Zhu, J Cao, Y Liu, Y Lu, Y Sun, Q Li, Y Huang, S Shang, X Bian, C Li, L Zhang, Y Wang, Y Nie, J Fu, W Li, MA Mazid, Y Jiang, W Jia, X Wang, Y Sun, MA Esteban, Q Sun, F Zhou, Z Liu Cynomolgus monkey embryo model captures gastrulation and early pregnancy Cell Stem Cell, 2023-04-06;30(4):362-377.e7. 2023-04-06 [PMID: 37028403] (Immunohistochemistry, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• AA Almad, L Garcia, A Takanohash, A Gagne, W Yang, J Ann McGuir, D French, A Vanderver Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G&gt;A (p.D249N) autosomal dominant mutation in TUBB4A Stem Cell Research, 2023-03-26;69(0):103083. 2023-03-26 [PMID: 37003180] (Flow Cytometry, ICC, Human)
• M Shigyo, Y Kobayashi, O Platoshyn, S Marsala, T Kato, N Takamura, K Yoshida, A Kishino, M Bravo-Hern, S Juhas, J Juhasova, H Studenovsk, V Proks, JD Ciacci, M Marsala Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization Cell Transplantation, 2023-01-01;32(0):9636897231163. 1/1/2023 [PMID: 36959733]
• AH Kim, HM Lee, HS Kim, J Jung, H Seol, E Choi, S Lee, Y Min Choi, JK Jun, HS Kim, J Jang Establishment of TUBB3-mCherry knock-in human pluripotent stem cell line using CRISPR/Cas9 (SNUe003-A-4) Stem Cell Research, 2023-03-08;69(0):103064. 2023-03-08 [PMID: 36913849] (Flow Cytometry, ICC, Human)
• Elena V. Grigor’eva, Alena E. Kopytova, Elena S. Yarkova, Sophia V. Pavlova, Diana A. Sorogina, Anastasia A. Malakhova et al. Biochemical Characteristics of iPSC-Derived Dopaminergic Neurons from N370S GBA Variant Carriers with and without Parkinson’s Disease International Journal of Molecular Sciences 2023-02-23 [PMID: 36901867]
• OJ Mainwaring, H Weishaupt, M Zhao, G Rosén, A Borgenvik, L Breinschmi, AD Verbaan, S Richardson, D Thompson, SC Clifford, RM Hill, K Annusver, A Sundström, KO Holmberg, M Kasper, S Hutter, FJ Swartling ARF suppression by MYC but not MYCN confers increased malignancy of aggressive pediatric brain tumors Nature Communications, 2023-03-03;14(1):1221. 2023-03-03 [PMID: 36869047] (ICC, Western Blot, Human)
• LR Bohrer, NE Stone, NK Mullin, AP Voigt, KR Anfinson, JL Fick, V Luangphakd, B Hittle, K Powell, GF Muschler, RF Mullins, EM Stone, BA Tucker Automating iPSC generation to enable autologous photoreceptor cell replacement therapy Journal of Translational Medicine, 2023-02-28;21(1):161. 2023-02-28 [PMID: 36855199] (IHC, Human)
• X Ma, S Han, Y Liu, Y Chen, P Li, X Liu, L Chang, YA Chen, F Chen, Q Hou, L Hou DAPL1 prevents epithelial-mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy Cell Death & Disease, 2023-02-25;14(2):158. 2023-02-25 [PMID: 36841807] (IHC, Mouse)
• Marion Déjosez, Arturo Marin, Graham M. Hughes, Ariadna E. Morales, Carlos Godoy-Parejo, Jonathan L. Gray et al. Bat pluripotent stem cells reveal unusual entanglement between host and viruses Cell 2023-03-02 [PMID: 36812912]
• C Dupont, OJM Schäffers, BF Tan, S Merzouk, EM Bindels, A Zwijsen, D Huylebroec, J Gribnau Efficient generation of ETX embryoids that recapitulate the entire window of murine egg cylinder development Science Advances, 2023-01-18;9(3):eadd2913. 2023-01-18 [PMID: 36652512] (IHC, Mouse)
• PH Zushin, Y Zhou, A Li, EA Ashley, MT Wheeler, JC Wu Generation of two human iPSC lines with Exon 3 mutations in BCL2-Associated Athanogene 3 (BAG3) from dilated cardiomyopathy patients Stem Cell Research, 2023-01-05;67(0):103019. 1/5/2023 [PMID: 36642055]
• S Adhicary, S Ye, H Lin, K Texter, V Garg, MT Zhao Establishment of NCHi009-A, an iPSC line from a patient with hypoplastic left heart syndrome (HLHS) carrying a heterozygous NOTCH1 mutation Stem Cell Research, 2022-12-31;66(0):103013. 12/31/2022 [PMID: 36599283]
• Ian Y. Chen, Joseph Olshausen, Dilip Thomas, Celine Lai, Tracey L. McLaughlin, Joseph C. Wu Generation of three induced pluripotent stem cell lines to model and investigate diseases affecting Hispanics Stem Cell Research 2022-12-01 [PMID: 36427473]
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Reviews for Otx2 Antibody (AF1979) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, pleurodeles waltl.

reviewed by: Verified Customer ICC pleurodeles waltl 03/30/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: brain
• Species: pleurodeles waltl
• Lot: KNO0919021

reviewed by: Verified Customer ICC Mouse 08/04/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: R1 wild type Mouse embryonic Stem Cells
• Species: Mouse
• Lot: KNO0615111

Bioinformatics

• Gene Symbol: OTX2
• Uniprot:
  • P32243()