OSTF1 Antibody (9G4) - BSA Free

Western Blot: OSTF1 Antibody (9G4) [NBP2-59396] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : MCF7 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : LnCap cell lysate

Immunocytochemistry/Immunofluorescence: OSTF1 Antibody (9G4) [NBP2-59396] - Analysis of OSTF1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of OSTF1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: OSTF1 Antibody (9G4) [NBP2-59396] - Analysis of OSTF1 in HeLa cell line, staining at 2-5ug for 1x10 6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

Western Blot: OSTF1 Antibody (9G4) [NBP2-59396] - Lane 1: 293T cell lysates, Lane 2: OSTF1 Transfected 293T cell lysates

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 9G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

OSTF1 Antibody (9G4) - BSA Free Summary

• Description: Novus Biologicals Mouse OSTF1 Antibody (9G4) - BSA Free (NBP2-59396) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human OSTF1 (1-217 aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OSTF1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1009
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for OSTF1 Antibody (9G4) - BSA Free

• FLJ20559
• OSFSH3P2bA235O14.1
• osteoclast stimulating factor 1
• osteoclast-stimulating factor 1

Background

Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OSTF1