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![Western Blot: OSTF1 Antibody [NBP2-13718] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/OSTF1-Antibody-Western-Blot-NBP2-13718-img0004.jpg "Western Blot: OSTF1 Antibody [NBP2-13718] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: OSTF1 Antibody [NBP2-13718] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/OSTF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13718-img0005.jpg)
![Immunohistochemistry-Paraffin: OSTF1 Antibody [NBP2-13718] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/OSTF1-Antibody-Immunohistochemistry-Paraffin-NBP2-13718-img0001.jpg)
![Western Blot: OSTF1 Antibody (9G4) [NBP2-59396] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : MCF7 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : LnCap cell lysate](http://images.novusbio.com/fullsize/OSTF1-Antibody-9G4-Western-Blot-NBP2-59396-img0005.jpg "Western Blot: OSTF1 Antibody (9G4) [NBP2-59396] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HepG2 cell lysate Lane 2 : MCF7 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : LnCap cell lysate")
![Immunocytochemistry/Immunofluorescence: OSTF1 Antibody (9G4) [NBP2-59396] - Analysis of OSTF1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of OSTF1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/OSTF1-Antibody-9G4-Immunocytochemistry-Immunofluorescence-NBP2-59396-img0002.jpg)
![Flow Cytometry: OSTF1 Antibody (9G4) [NBP2-59396] - Analysis of OSTF1 in HeLa cell line, staining at 2-5ug for 1x10 6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/OSTF1-Antibody-9G4-Flow-Cytometry-NBP2-59396-img0003.jpg)
![Western Blot: OSTF1 Antibody (9G4) [NBP2-59396] - Lane 1: 293T cell lysates, Lane 2: OSTF1 Transfected 293T cell lysates](http://images.novusbio.com/fullsize/OSTF1-Antibody-9G4-Western-Blot-NBP2-59396-img0001.jpg)
![Western Blot: OSTF1 Antibody [H00026578-B01P] - Analysis of OSTF1 expression in transfected 293T cell line by OSTF1 polyclonal antibody. Lane 1: OSTF1 transfected lysate(23.98 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/OSTF1-Antibody-Western-Blot-H00026578-B01P-img0003.jpg "Western Blot: OSTF1 Antibody [H00026578-B01P] - Analysis of OSTF1 expression in transfected 293T cell line by OSTF1 polyclonal antibody. Lane 1: OSTF1 transfected lysate(23.98 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: OSTF1 Antibody [H00026578-B01P] - Analysis of purified antibody to OSTF1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/OSTF1-Antibody-Immunocytochemistry-Immunofluorescence-H00026578-B01P-img0002.jpg)
![Western Blot: OSTF1 Antibody [H00026578-B02P] - Analysis of OSTF1 expression in transfected 293T cell line by OSTF1 polyclonal antibody. Lane 1: OSTF1 transfected lysate(23.87 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/OSTF1-Antibody-Western-Blot-H00026578-B02P-img0004.jpg "Western Blot: OSTF1 Antibody [H00026578-B02P] - Analysis of OSTF1 expression in transfected 293T cell line by OSTF1 polyclonal antibody. Lane 1: OSTF1 transfected lysate(23.87 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: OSTF1 Antibody [H00026578-B02P] - Analysis of purified antibody to OSTF1 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/OSTF1-Antibody-Immunocytochemistry-Immunofluorescence-H00026578-B02P-img0003.jpg)
![Western Blot: OSTF1 Antibody [NBP1-50036] - Detection of Human OSTF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-OSTF1 antibody used for IP at 6 mcg/mg lysate. OSTF1 was also immunoprecipitated by rabbit anti-OSTF1 antibody BL10922, which recognizes an upstream epitope. For blotting immunoprecipitated OSTF1 was used at 1 mcg/ml, and the ReliaBLOT(R) Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/OSTF1-Antibody-Western-Blot-NBP1-50036-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: OSTF1 Antibody [NBP1-50036] - Detection of Human OSTF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-OSTF1 antibody used for IP at 6 mcg/mg lysate. OSTF1 was also immunoprecipitated by rabbit anti-OSTF1 antibody BL10922, which recognizes an upstream epitope. For blotting immunoprecipitated OSTF1 was used at 1 mcg/ml, and the ReliaBLOT(R) Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![ELISA: Human OSTF1 ELISA Kit (Colorimetric) [NBP2-76786] - Samples were spiked with high concentrations of Human OSTF1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76786_human-ostf1-elisa-kit-colorimetric-132202416173430.jpg "ELISA: Human OSTF1 ELISA Kit (Colorimetric) [NBP2-76786] - Samples were spiked with high concentrations of Human OSTF1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human OSTF1 ELISA Kit (Colorimetric) [NBP2-76786] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76786_human-ostf1-elisa-kit-colorimetric-4320249124883.jpg)
![ELISA: Rat OSTF1 ELISA Kit (Colorimetric) [NBP2-76787] - Samples were spiked with high concentrations of Rat OSTF1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76787_rat-ostf1-elisa-kit-colorimetric-132202416225222.jpg "ELISA: Rat OSTF1 ELISA Kit (Colorimetric) [NBP2-76787] - Samples were spiked with high concentrations of Rat OSTF1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat OSTF1 ELISA Kit (Colorimetric) [NBP2-76787] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76787_rat-ostf1-elisa-kit-colorimetric-432024919286.jpg)
![Western Blot: OSTF1 Overexpression Lysate (Adult Normal) [NBP2-07456] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OSTF1.](http://images.novusbio.com/fullsize/OSTF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07456-img0001.jpg "Western Blot: OSTF1 Overexpression Lysate (Adult Normal) [NBP2-07456] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OSTF1.")
![Sandwich ELISA: OSTF1 Antibody (2E6) [H00026578-M06] - Detection limit for recombinant GST tagged OSTF1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/OSTF1-Antibody-2E6-Sandwich-ELISA-H00026578-M06-img0001.jpg "Sandwich ELISA: OSTF1 Antibody (2E6) [H00026578-M06] - Detection limit for recombinant GST tagged OSTF1 is 0.03 ng/ml as a capture antibody.")
![Western Blot: OSTF1 Protein [NBC1-19070] - 25.1 kDa (225 aa) on SDS - PAGE](http://images.novusbio.com/fullsize/NBC1-19070.jpg "Western Blot: OSTF1 Protein [NBC1-19070] - 25.1 kDa (225 aa) on SDS - PAGE")