| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF |
| Clone | 9G4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | FITC |
| Immunogen | Recombinant human OSTF1 (1-217 aa) purified from E. coli |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | OSTF1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Endometrium tissue within the normal limits using antiMAPK1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min,Dilution 1:50)](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Immunohistochemistry-Paraffin-NBP1-47842-img0019.jpg)
![Immunoprecipitation: ERK2 Antibody (6E5) [NBP1-47842] - TrueMab monoclonal anti-ERK2 antibody (Negative control: IP without adding anti-ERK2 antibody). For each experiment, 500ul of DDK tagged ERK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of antibody.](http://images.novusbio.com/images2/ERK2-Antibody-(6E5)-Immunoprecipitation-NBP1-47842-img0023.jpg)
![Immunohistochemistry-Paraffin: ERK2 Antibody (6E5) [NBP1-47842] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-ERK2 mouse monoclonal antibody.](http://images.novusbio.com/images/ERK2-Antibody-(6E5)-Immunohistochemistry-Paraffin-NBP1-47842-img0003.jpg)
![Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).](http://images.novusbio.com/images2/GST-Epitope-Tag-Antibody-Western-Blot-NB600-326-img0001.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Simple Western lane view shows a specific band for GST Epitope Tag in 5 ng/ml of hUbiquitn-GST (left) & hISG15-GST (right) lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/images2/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0004.jpg)
![Simple Western: GST Epitope Tag Antibody [NB600-326] - Electropherogram image(s) of corresponding Simple Western lane view. GST Epitope Tag antibody was used at 1:2000 dilution on hUbiquitn-GST (left) & hISG15-GST (right) lysate(s).](http://images.novusbio.com/images2/GST-Epitope-Tag-Antibody-Simple-Western-NB600-326-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Immunocytochemistry-Immunofluorescence-NB500-106-img0010.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Western-Blot-NB500-106-img0011.jpg)
![Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/images2/PCNA-Antibody-(PC10)-Flow-Cytometry-NB500-106-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: LOX Antibody [NB100-2527] - Staining in human fibroblasts. Image from verified customer review.](http://images.novusbio.com/images2/LOX-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2527-img0013.jpg)
![Immunohistochemistry: LOX Antibody [NB100-2527] - IR-enhanced LOX in tumor xenografts. Tumor tissue from A549-derived tumor xenografts treated with 1 x 10 Gy and 2 x 10 Gy (with 12 h between fractions) and evaluated by immunohistochemistry for LOX at different time points after irradiation. Whole tumor sections were quantified for specific LOX-staining intensity. Each treatment group consists of 3 animals. At least 3 sections per tumor/animal were analyzed. Image collected and cropped by CiteAb from the following publication (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY licence.](http://images.novusbio.com/images2/LOX-Antibody-Immunohistochemistry-NB100-2527-img0014.jpg)
![Western Blot: LOX Antibody [NB100-2527] - LOX secretion and activity in response to tumor cell irradiation. Western blot of LOX zymogen (50 kDa) and active form (32 kDa) in conditioned media derived from irradiated A549 lung carcinoma cells 16-20 h after irradiation and quantification of band intensities from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (http://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-532) licensed under a CC-BY licence.](http://images.novusbio.com/images2/LOX-Antibody-Western-Blot-NB100-2527-img0015.jpg)
![OSTF1 Antibody (9G4) [FITC]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "OSTF1 Antibody (9G4) [FITC]")
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | OSTF1 |
