DNA Polymerase gamma Antibody

Images

 
Western Blot: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with (30 ug of whole cell lysate). A: 293T. 5% SDS PAGE. POLG antibody diluted at 1:5000.
Immunocytochemistry/ Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with methanol-fixed HeLa cell at 1:200 dilution.
Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with human skeletal muscle at concentration 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml

Order Details

DNA Polymerase gamma Antibody Summary

Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA polymerase gamma.
Specificity
Human DNA polymerase gamma. Predicted cross-reactivity based on amino acid sequence homology: mouse (91%), rat (91%).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry-Paraffin 10 ug/ml
Theoretical MW
139.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-52300 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DNA Polymerase gamma Antibody

  • DNA polymerase subunit gamma-1
  • EC 2.7.7.7
  • FLJ27114
  • MDP1
  • MIRAS
  • Mitochondrial DNA polymerase catalytic subunit
  • MTDPS4A
  • PolG, catalytic subunit
  • POLG1MTDPS4B
  • PolG-alpha
  • POLGAPEO
  • polymerase (DNA directed), gamma
  • SANDO
  • SCAE

Background

As the only DNA polymerase (pol) present in mitochondria, DNA polymerase gamma (POLG) is necessarily implicated in base excision repair processes to correct oxidative damage to the mitochondrial genome. Therefore, the ability of the catalytic subunit of human POLG has been tested to participate in uracil-provoked base excision repair reconstituted in vitro with purified components. Subsequent to actions of uracil-DNA glycosylase and apurinic/apyrimidinic endonuclease, human POLG is able to fill a single nucleotide gap in the presence of a 5' terminal deoxyribose phosphate (dRP) flap. The catalytic subunit of human pol gamma catalyzes release of the dRP residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. Faulty replication of the human mitochondrial genome is thought to be the cause of many diseases. The low selectivity of the mitochondrial DNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DNA Polymerase gamma Antibody (NBP1-52300)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DNA Polymerase gamma Antibody (NBP1-52300). (Showing 1 - 1 of 1 FAQs).

  1. I am planning on using your antibody NBP1-52300 and am wondering about the immunogen. I understand that you might not be able to give me the exact sequence, but it is of great importance to me that the antibody does not bind to amino acids 994-1040. Is it possible for you to answer whether the antibody binds to this position or not?
    • The antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 829 and 1201 of DNA polymerase gamma. We typically do not epitope map our antibodies and I cannot determine if this antibody does or does not bind to amino acids 994-1040 however the immunogen sequence does include that fragment.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol POLG
Entrez
Uniprot